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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616534/
Through concurrent delivery of a novel lentiviral reporter vector (lenti-NF-κB-luc-GFP) we track in vivo expression of NF-κB target genes in real time, a critical step towards extending RNAi-based therapy to longstanding lung diseases. Application of this system reveals that resident AMs persist in the airspaces of mice following the resolution of LPS-induced inflammation, thus allowing these localized cells to be …Author: Andrew A Wilson, Andrew A Wilson, Letty W Kwok, Letty W Kwok, Emily L Porter, Emily L Porter, Julie ...
https://www.ncbi.nlm.nih.gov/pubmed/23403494
Through concurrent delivery of a novel lentiviral reporter vector (lenti-NF-κB-luc-GFP) we track in vivo expression of NF-κB target genes in real time, a critical step towards extending RNAi-based therapy to longstanding lung diseases.Author: Andrew A Wilson, Andrew A Wilson, Letty W Kwok, Letty W Kwok, Emily L Porter, Emily L Porter, Julie ...
https://www.addgene.org/browse/article/7349/
Lentiviral delivery of RNAi for in vivo lineage-specific modulation of gene expression in mouse lung macrophages. Wilson AA, Kwok LW, Porter EL, Payne JG, McElroy GS, Ohle SJ, Greenhill SR, Blahna MT, Yamamoto K, Jean JC, Mizgerd JP, Kotton DN
https://www.pnas.org/content/103/31/11479
Aug 01, 2006 · This demonstration of targeting efficient gene delivery vehicles strictly to the desired cell types in vivo greatly enhances the therapeutic potential of lentivirus-mediated gene therapy and alleviates concerns of off-target effects. Possibly the most important implication of the work is that gene therapy could now be carried out as an inexpensive procedure, able to be considered even in the less …Author: Lili Yang, Leslie Bailey, David Baltimore, Pin Wang
https://www.sigmaaldrich.com/life-science/functional-genomics-and-rnai/shrna/in-vivo.html
MISSION in vivo lentivirus is designed to address the challenges of extending an RNAi study into an in vivo system. Lentivirus-delivered shRNAs in vivo has been accomplished by either single high-titer injections, or serial lower-dose administration. MISSION in vivo lentivirus is suitable for RNAi research in animals using multiple routes of delivery.
https://www.sciencedirect.com/science/article/pii/S2162253117302445
In support of this notion, we show that Streptococcus pyogenes (Sp) Cas9, delivered by lentiviral vectors (LVs), can be used in vivo to selectively ablate the vascular endothelial growth factor A …
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