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https://www.labome.com/method/Nucleic-Acid-Delivery-Lentiviral-and-Retroviral-Vectors.html
Lentiviral and retroviral gene delivery systems exploit aspects of retrovirus replication to provide stable integration of the desired nucleic acid sequence.Author: Erin P O’Keefe
http://www.eurekaselect.com/142590/article
By now, the efficacy of lentiviral gene delivery to stem cells, cells of the immune system including T cells, hepatic cells, and many other therapeutically relevant cell types is well established. Along with nucleic acids, HIV-1 virions carry the enzymatic tools that are essential for early steps of infection.Author: Yujia Cai, Jacob Giehm Mikkelsen
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2789654/
Jul 25, 2009 · 5. Lentiviral shRNA delivery for viral infections. Although lentivirally expressed shRNAs have also been evaluated for potential therapeutic application in cancer [127–129] and neurodegenerative diseases (reviewed in [130–132]) in this review, we …Author: N. Manjunath, Haoquan Wu, Sandesh Subramanya, Premlata Shankar
https://www.nature.com/articles/s41375-018-0106-0
Mar 22, 2018 · In vivo gene delivery using a lentiviral vector has also been applied clinically to the eye . These approaches face a number of hurdles including efficiency, need for tissue-restricted promoters ...Author: Michael C. Milone, Una O’Doherty
https://www.addgene.org/guides/lentivirus/
To increase the safety of lentivirus, the components necessary for virus production are split across multiple plasmids (3 for 2nd-generation systems, 4 for 3rd-generation systems). The components of both systems are as follows: Lentiviral transfer plasmid encoding your insert of interest.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · The gene delivery efficiency of liposomes is dependent on the size, structure, and even the amount of the liposome, the charge ratio between transgenic DNA and cationic liposome, presence of helper lipid, and the structure and proportion of it and cell type.
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