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https://www.sciencedirect.com/science/article/pii/S1525001604001030
In principle, anionic proteins should be amenable to intracytoplasmic delivery by cationic vectors, provided some endosomal escape is possible. Cationic lipids form multilamellar sandwich complexes with polyanionic macromolecules [12 ]. Among them, lipospermines [ 13] …Author: Deniz Dalkara, Guy Zuber, Jean-Paul Behr
https://www.researchgate.net/publication/8513576_Intracytoplasmic_Delivery_of_Anionic_Proteins
Intracytoplasmic Delivery of Anionic Proteins*. Protein delivery is emerging as an interesting alternative to gene delivery. We have used our experience with transfection to develop a technique for efficient delivery of anionic proteins such as antibodies into the cytoplasm of cells.
https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S2162-2531(16)30029-4
Jan 01, 2015 · The intracellular delivery of biologically active protein represents an important emerging strategy for both fundamental and therapeutic applications. Here, we optimized in vitro delivery of two functional proteins, the β-galactosidase (β-gal) enzyme and the anti-cytokeratin8 (K8) antibody, using liposome-based formulation. The guanidinium-cholesterol cationic lipid bis (guanidinium)-tren …Author: Benoît Chatin, Benoît Chatin, Mathieu Mével, Mathieu Mével, Julie Devallière, Julie Devallière, Laur...
https://www.ncbi.nlm.nih.gov/pubmed/31686002
Anionic nanoparticles enable the oral delivery of proteins by enhancing intestinal permeability. Lamson NG(1), Berger A(1)(2), Fein KC(1), Whitehead KA(3)(4). Author information: (1)Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.Author: Nicholas G. Lamson, Adrian Berger, Katherine C. Fein, Kathryn A. Whitehead
https://www.nature.com/articles/nchem.2779
May 22, 2017 · The delivery peptides were developed by introducing one or two glutamic acid residues into the hydrophobic face. One peptide with the substitution of leucine by glutamic acid (L17E) was shown to enable a marked cytosolic liberation of antibodies (immunoglobulins G …Author: Misao Akishiba, Toshihide Takeuchi, Yoshimasa Kawaguchi, Kentarou Sakamoto, Hao-Hsin Yu, Ikuhiko Nak...
https://www.sciencedirect.com/science/article/pii/S2162253116300294
Intracellular delivery of functional proteins provides a potential tool to replace missing, dysfunctional and poorly expressed proteins or antagonize key intracellular pathways. Protein-based therapeutics is a promising approach for several biomedical applications including stem cell engineering, imaging, and signaling studies.Author: Benoît Chatin, Benoît Chatin, Mathieu Mével, Mathieu Mével, Julie Devallière, Julie Devallière, Laur...
https://mct.aacrjournals.org/content/6/6/1728
Jun 01, 2007 · Recently, it has been shown that certain cationic lipids can be used for the intracytoplasmic delivery of proteins, including antibodies (16–18). Because cervical cell lines can be transfected readily with siRNA, we investigated whether lipid-based reagents used for siRNA transfection would also be effective for the intracellular delivery of anti-E6 antibodies.Author: Jérôme Courtête, Annie-Paule Sibler, Gabrielle Zeder-Lutz, Deniz Dalkara, Mustapha Oulad-Abdelghani,...
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