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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064722/
Hydrodynamic delivery is one of non-viral methods developed originally for intracellular gene delivery but later found applicable to delivery of other macromolecules such as oligo nucleotides, RNA, proteins, and the compounds that are not permeable to cell membrane.Author: Barbara Bonamassa, Li Hai, Dexi Liu
https://www.sciencedirect.com/science/article/pii/S1525001616327198
More recently, hydrodynamic delivery has been used for delivery of small interfering RNA (siRNA) for target validation and gene function studies. The first clinical test of hydrodynamic gene delivery in humans was reported at the 9th Annual Meeting of the American Society of Gene Therapy. 4Author: Takeshi Suda, Dexi Liu
https://www.sciencedirect.com/science/article/pii/S0065266005540045
Because of its simplicity, convenience, high efficiency, and lack of toxicity to animals, the hydrodynamics‐based procedure (now more often referred to as hydrodynamic delivery or hydrodynamic injection) has become a frequent choice for the delivery of DNA and RNA (especially siRNA) for gene function and gene therapy studies in mice.
https://link.springer.com/protocol/10.1007%2F978-1-59745-191-8_5
We describe the use of hydrodynamic administration as a technique to deliver, in vivo, naked siRNA constructs into experimental animals as a method of transient gene knockdown. This method may prove useful in situations where knockout animals do not exist, or to determine the effect of gene knockdown at specific time points during an experiment.Author: Doreen E. Wesche-Soldato, Joanne Lomas-Neira, Mario Perl, Chun-Shiang Chung, Alfred Ayala
https://www.researchgate.net/publication/5482383_Hydrodynamic_Delivery_of_siRNA_in_a_Mouse_Model_of_Sepsis
Hydrodynamic gene delivery has proven to be a safe and efficient procedure for gene transfer, able to mediate, in murine model, therapeutic levels of proteins encoded by the transfected gene. In...
https://www.mirusbio.com/tech-resources/tips/deliver-sirna-in-vivo
siRNA is effectively delivered to the mouse liver using hydrodynamic tail vein injection and TransIT®-QR Delivery Solution. Given the similar size and structure of siRNAs and duplex miRNAs, this method should also deliver duplex miRNAs to the mouse liver with similar efficiency.
https://www.thermofisher.com/us/en/home/references/ambion-tech-support/rnai-sirna/tech-notes/getting-started-with-rnai-in-vivo.html
In this article, we describe siRNA delivery to the liver of mice by hydrodynamic (high-pressure) tail vein injection [4]. Because a large volume (equal to blood volume) is intravenously injected within a few seconds, contact time of siRNA with blood is limited, and the siRNA is almost quantitatively and specifically delivered to liver cells.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198622/
We investigated whether immune activation as a consequence of double-stranded RNA induced IFN response (Jak/STAT pathway activation or cytokine production) or target organ damage is induced by in vivo low-volume (LV) or high-volume (HV) hydrodynamic delivery or treatment with naked siRNA. NMRI mice were injected with naked siRNAs or saline by ...Author: Zsuzsanna Rácz, Mária Godó, Csaba Révész, Péter Hamar
https://www.mirusbio.com/products/in-vivo/transit-qr-delivery-solution
Versatile Platform– for DNA or siRNA delivery using hydrodynamic tail vein injection to the mouse strain of your choice Low Toxicity – Minimized loss of cardiac output compared to saline injections, which allows for quick recovery (within minutes) of the mouse post-injection
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