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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064722/
Hydrodynamic delivery has emerged as the simplest and most effective method for intracellular delivery of membrane impermeable substances in rodents. The system employs a physical force generated by a rapid injection of large volume of solution into a ...Author: Barbara Bonamassa, Li Hai, Dexi Liu
https://www.sciencedirect.com/science/article/pii/S1525001616327198
Hydrodynamic gene delivery has been shown to be able to promote both humoral- and cell-based immunity in animals in a gene dependent manner. Hydrodynamic gene delivery generates much more gene product than does the commonly used method of intramuscle injection of plasmid DNA.Author: Takeshi Suda, Dexi Liu
https://www.sciencedirect.com/science/article/pii/S0065266005540045
Hydrodynamic delivery has emerged as a near‐perfect method for intracellular DNA delivery in vivo.For gene delivery to parenchymal cells, only essential DNA sequences need to be injected via a selected blood vessel, eliminating safety concerns associated with current viral and synthetic vectors.
https://en.wikipedia.org/wiki/Hydrodynamic_technique
Hydrodynamic voltammetry is a form of voltammetry in which the analyte solution flows relative to a working electrode. In many voltammetry techniques, the solution is intentionally left still to allow diffusion controlled mass transfer.When a solution is made to flow, through stirring or some other physical mechanism, it is very important to the technique to achieve a very controlled flux or ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4096022/
The hydrodynamic tail vein injection is a technique that is used to deliver nucleic acids into live mice. Delivery through this method results in the in vivo transfection of foreign DNA primarily in the liver. Here, we describe the use of this technique ...Author: Mee J. Kim, Nadav Ahituv
https://www.researchgate.net/publication/7664463_Hydrodynamic_Delivery
The hydrodynamic method by rapid intravenous injection of a large volume of plasmid DNA is known to be an efficient and liver-specific method of in vivo gene delivery and achieves high levels of ...
https://link.springer.com/protocol/10.1007%2F978-1-4939-3581-9_9
Apr 24, 2016 · Abstract. Gene delivery methods are important for both therapeutic intervention and as tools in research to address specific questions. Hydrodynamic injection (HDI) is a method that facilitates the delivery and expression of genetic material in target cells, namely hepatocytes, through an intravenous injection.Author: Simon P. Preston, Simon P. Preston, Marc Pellegrini, Marc Pellegrini, Gregor Ebert, Gregor Ebert
https://www.frontiersin.org/articles/10.3389/fphar.2017.00591/full
Aug 30, 2017 · Development of an safe and efficient in vivo gene delivery method is indispensable for molecular biology research and the progress in the following gene therapy. Over the past few years, hydrodynamic gene delivery (HGD) with naked DNA has drawn increasing interest in both research and potential clinic applications due to its high efficiency and low risk in triggering immune responses and ...Author: Mei Huang, Rui Sun, Qiang Huang, Zhigang Tian
https://www.jove.com/video/51481/transient-expression-of-proteins-by-hydrodynamic-gene-delivery-in-mice
In addition, since the DNA molecules delivered by HGD do not need packaging, this method is suitable for the delivery of bacterial artificial chromosomes (BAC) as large as 150 kb 4. Other types of molecules that have been delivered by a hydrodynamic method include RNA 5-10, morpholinos 11, proteins 12, 13 and other small molecules 12, 14.Author: Daniella Kovacsics, Jayne Raper
https://www.nature.com/articles/3302891
Nov 30, 2006 · Over the last few years, hydrodynamic tail vein delivery has established itself as a simple, yet very effective method for gene transfer into small rodents. Hydrodynamic delivery of plasmid DNA ...Author: H Herweijer, J A Wolff, J A Wolff
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