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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064722/
Hydrodynamic delivery has emerged as the simplest and most effective method for intracellular delivery of membrane impermeable substances in rodents. The system employs a physical force generated by a rapid injection of large volume of solution into a blood vessel to enhance the permeability of endothelium and the plasma membrane of the parenchyma cells to allow delivery of substance into cells.Author: Barbara Bonamassa, Li Hai, Dexi Liu
https://www.mirusbio.com/products/in-vivo/transit-qr-delivery-solution
TransIT®-QR Delivery Solution was used to deliver 25 µg of Label IT® Cy®3 RNAi Delivery Control (red) to a mouse using hydrodynamic delivery via the tail vein. Forty-five minutes post-injection the liver was harvested and sections were fixed then counterstained to stain the nuclei (blue) and to …
https://www.sciencedirect.com/science/article/pii/S0065266005540045
Hydrodynamic delivery employs the force generated by the rapid injection of a large volume of solution into the incompressible blood in the circulation to overcome the physical barriers of endothelium and cell membranes that prevent large and membrane‐impermeable compounds from entering parenchymal cells.
https://www.fishersci.com/shop/products/mirus-bio-i-trans-i-it-ee-hydrodynamic-delivery-solution-starter-kit-2/p-3082126
Delivery of DNA expression vectors into live laboratory mice using the hydrodynamic tail vein injection procedure View More Specs Includes: Provided as Trans IT-EE Delivery Solution or Trans IT-EE Starter Kit including the Trans IT-EE Delivery Solution, 10 syringes, needles and alcohol swabs and conical plastic mouse restraint device.
https://www.sciencedirect.com/science/article/pii/S1525001616327198
Hydrodynamic gene delivery uses a hydrodynamic force generated by a pressurized injection of a large volume of DNA solution into the blood vessel so as to permeabilize the capillary endothelium and generate “pores” in the plasma membrane of the surrounding parenchyma cells, through which DNA or other macromolecules of interest may reach the cell interior. 35, 36, 37 With time, the membrane …Author: Takeshi Suda, Dexi Liu
https://www.researchgate.net/publication/271535808_Hydrodynamic_Delivery
Hydrodynamic delivery (HD) is a broadly used procedure for DNA and RNA delivery in rodents, serving as a powerful tool for gene/protein drug discovery, gene function analysis, target validation,...
https://www.researchgate.net/publication/5933252_Hydrodynamic_Gene_Delivery_Its_Principles_and_Applications
Recently, hydrodynamic gene delivery using a rapid injection of a relatively large volume of DNA solution has opened up a new avenue for gene therapy studies in vivo. This method is superior to the existing delivery systems because of its simplicity, efficiency, and versatility.
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