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https://www.sciencedirect.com/science/article/pii/B9780123849649000037
Gene delivery using nonviral methods is an exciting research area that has been explored for intracellular transgene expression in gene therapy. Nonviral gene delivery is achieved using both physical and chemical methods for safe and efficient transgene expression in target cells.Author: Kaustubh A. Jinturkar, Mohan N. Rathi, Ambikanandan Misra
https://link.springer.com/protocol/10.1385/1-59259-649-5:147
Abstract. The study of gene transfer has always been a major component of modern molecular biology. Initially, transfer of exogenous genes into cells was considered a critical technical step toward understanding the function and regulation of newly discovered genes.Author: Te-hui W. Chou, Subhabrata Biswas, Shan Lu
https://www.sciencedirect.com/topics/neuroscience/gene-delivery
Gene delivery using nonviral methods is an exciting research area that has been explored for intracellular transgene expression in gene therapy. Nonviral gene delivery is achieved using both physical and chemical methods for safe and efficient transgene expression in target cells.
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/transfection-basics/gene-delivery-technologies.html
To sneak nucleic acids through the cell membrane, researchers have developed a number of techniques each using a different approach—from using chemicals and carrier molecules that coat the nucleic acids to neutralize them to physical methods that create transient pores in the membrane to introduce the DNA directly into the cell.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · Physical methods of nonviral gene delivery. Physical methods applied for in vitro and in vivo gene delivery are based on making transient penetration in cell membrane by mechanical, electrical, ultrasonic, hydrodynamic, or laser-based energy so that DNA entrance into the targeted cells is facilitated.
https://www.slideshare.net/Aparajitha_Anne/gene-delivery-system
May 19, 2012 · Gene Vectors can make from the HSV in 2 methods:Gene Vectors can make from the HSV in 2 methods:1. Cloning therapeutic gene into plasmid which contains HSV and can be packed in the cells and infects with the help of HSV.2. Cloning gene into a plasmid which was surrounded by HSV sequences andco transfected to cells with HSV. 16.
https://www.ncbi.nlm.nih.gov/pubmed/14707376
Gene delivery using physical methods: an overview. Chou TH(1), Biswas S, Lu S. Author information: (1)Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.Author: Te-hui W. Chou, Subhabrata Biswas, Shan Lu
https://seekingalpha.com/article/4305556-gene-therapy-series-2-delivery-systems-in-gene-therapy-including-viral-vectors
Nov 11, 2019 · Examples of gene therapy methods using ex vivo delivery include CAR-T therapies like Yescarta and Kymriah. In vivo delivery: By definition, this means inside the body. In …
https://link.springer.com/chapter/10.1007%2F978-3-319-53457-2_6
May 24, 2017 · However, the immunogenic and mutagenic side effects of viral vector as well as toxicity and low efficacy of nonviral carriers are limiting their application. In this respect, naked NAs delivery by physical methods could be the safest procedure for gene therapy strategies if …Author: María José Herrero, Luis Sendra, Antonio Miguel, Salvador F. Aliño, Salvador F. Aliño
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