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https://www.sutter.com/PDFs/RP0028_King-2.pdf
Gene Delivery to Mammalian Cells by Microinjection Robert King 1. Introduction Microinjection—that is, the direct-pressure injection of a solution into a cell through a glass capillary—is an effective and reproducible method for intro-ducing exogenous material into …
https://link.springer.com/protocol/10.1385/1-59259-649-5:167
Barber (1, 2) first described the technique that forms the basis for today’s microinjection applications. Described here is a simple method for microinjecting individual adherent cells in culture. This application may be used for microinjecting the myriad mammalian cell types that may be encountered in this field.Author: Robert King
https://www.researchgate.net/publication/8933364_Gene_Delivery_to_Mammalian_Cells_by_Microinjection
Microinjection is a powerful tool to deliver various substances, such as nucleic acids, proteins, peptides, RNA, and synthetic molecules into mammalian cells mechanically.
https://www.springer.com/gp/book/9781588290861
Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.
https://www.sciencedirect.com/topics/medicine-and-dentistry/microinjection
Conceptually, microinjection is the simplest gene delivery method. However, it is difficult to apply. Although pronuclear injection of DNA is very efficient, it is a laborious procedure; only one cell at a time can be injected, typically allowing for only a few hundred cells to be transfected per experiment.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642230/
Although microinjection has been well established for cellular delivery 20,21,22,23,24,25,26,27,28,29,30,31, our study is the first to demonstrate its use for delivering gene editing reagents into human somatic cells and achieve a high level of gene modification, including gene insertion. However, although we were able to microinject up to 1000 ...Author: Renee N. Cottle, Ciaran M. Lee, David Archer, Gang Bao
https://www.nature.com/articles/srep16031
Nov 12, 2015 · Controlled delivery of β-globin-targeting TALENs and CRISPR/Cas9 into mammalian cells for genome editing using microinjection Skip to main content Thank you for visiting nature.com.Author: Renee N. Cottle, Ciaran M. Lee, David Archer, Gang Bao
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · INTRODUCTION. Basically gene therapy is an intracellular delivery of genomic materials (transgene) into specific cells to generate a therapeutic effect by correcting an existing abnormality or providing the cells with a new function.[] Different types of gene delivery systems may be applied in gene therapy to restore a specific gene function or turning off a special gene(s).
https://link.springer.com/book/10.1385%2F1592596495
Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.
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