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https://www.intechopen.com/books/recent-advances-in-novel-drug-carrier-systems/gene-delivery-systems-recent-progress-in-viral-and-non-viral-therapy
The use of non-viral gene delivery systems in gene therapy also significantly increased in recent years. Naked plasmid DNA, coated with gold particles was effectively introduced to cells using the non-viral gene gun technique.Author: Erdal Cevher, Ali Demir Sezer, Emre Şefik Çağlar
https://cdn.intechopen.com/pdfs/40265/InTech-Gene_delivery_systems_recent_progress_in_viral_and_non_viral_therapy.pdf
Gene Delivery Systems: Recent Progress in Viral and Non-Viral Therapy 439 development of retroviral vectors (Yaron et al., 1997). In the mid-1980s, gene transfer to mammalian cells became a routine procedure. Retrovirus-based gene therapies brought significant advantages, as they can stably integrate their genomes to host-cell chromosomes.
https://www.researchgate.net/publication/257924981_Gene_Delivery_Systems_Recent_Progress_in_Viral_and_Non-Viral_Therapy
Gene Delivery Systems: Recent Progress in Viral and Non-Viral Therapy 459 The aim of cationic lipid-based gene therapy is to transport plasmid DNA to the cell and thus to achieve the desired ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782077/
Oct 16, 2009 · The nonviral gene delivery methods, on the other hand, use synthetic or natural compounds or physical forces to deliver a piece of DNA into a cell. The materials used are generally less toxic and immunogenic than the viral counterparts.Author: Mohammed S. Al-Dosari, Xiang Gao
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · Although numerous viral and nonviral gene delivery systems have been developed in the last 3 decades, no delivery system has been designed that can be applied in gene therapy of all kinds of cell types in vitro and in vivo with no limitation and side effects.
https://www.nature.com/articles/3301923
Nov 29, 2002 · Gene Therapy Progress and Prospects: Nonviral vectors ... a great deal of attention in recent years. Nonviral vectors should circumvent some of the problems occurring with viral …Author: T Niidome, L Huang
https://www.ncbi.nlm.nih.gov/pubmed/24164239
Therefore, the development of safe, effective, and clinically applicable gene delivery techniques for selected nonviral and viral vectors will certainly be invaluable in obtaining future regulatory approvals. The choice of gene transfer vector, dose level, and the delivery system are likely to be critical determinants of therapeutic efficacy.Author: Michael G. Katz, Anthony S. Fargnoli, Richard D. Williams, Charles R. Bridges
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814977/
The main benefits of nonviral gene therapy targeting the cardiovascular system are (1) very low risk for an adaptive immune response, (2) low toxicity, (3) ease of production, (4) relative simplicity, and (5) increased flexibility in applying recombinant techniques for enhancing cardiac specificity with promoters and also incorporating larger expression cassettes.Author: Michael G. Katz, Anthony S. Fargnoli, Richard D. Williams, Charles R. Bridges
https://www.intechopen.com/books/gene-therapy-tools-and-potential-applications/non-viral-delivery-systems-in-gene-therapy
Gene delivery systems include viral vectors and non-viral vectors. Viral vectors are the most effective, but their application is limited by their immunogenicity, oncogenicity and the small size of the DNA they can transport. Non-viral vectors …Author: Alicia Rodríguez Gascón, Ana del Pozo-Rodríguez, María Ángeles Solinís
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