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http://www.biologydiscussion.com/gene/therapy/gene-therapy-ex-vivo-and-in-vivo-gene-therapy-with-diagram/9969
The direct delivery of genes into the cells of a particular tissue is referred to as in vivo gene therapy. Type # I. Ex Vivo Gene Therapy: The ex vivo gene therapy can be applied to only selected tissues (e.g., bone marrow) whose cells can be cultured in the laboratory. The technique of ex vivo gene therapy involves the following steps (Fig. 13 ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · Physical methods of nonviral gene delivery. Physical methods applied for in vitro and in vivo gene delivery are based on making transient penetration in cell membrane by mechanical, electrical, ultrasonic, hydrodynamic, or laser-based energy so that DNA entrance into the targeted cells is facilitated.
http://lib.store.yahoo.net/lib/yhst-131428861332406/How-to-Choose-the-Optimal-Gene-Delivery-Method.pdf
polyethylenimine (PEI), as well as viral and mechanical gene delivery methods. We will now review the benefits and drawbacks of each gene transfer method to clarify the applications for which each method is most appropriate. 3. Chemical Methods of Gene Delivery 3.1. DEAE-dextran - Diethylaminoethyl-dextran (DEAE-Dextran) is a polycatonic ...
https://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/transfection-support/gene-delivery-selection-guide.html
Researchers have a wide variety of gene delivery techniques to introduce plasmid DNA, siRNA or duplex RNAi, oligonucleotides, and RNA into eukaryotic cells for a …
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/transfection-basics/gene-delivery-technologies.html
Gene delivery technologies Transfection technologies available today can be broadly classified into three groups: chemical, biological, and physical. No one method can be applied to all cells and all experiments.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722627/
Although gene therapy as a treatment for disease holds great promise, progress in developing effective clinical protocols has been slow. The problem lies in the development of safe and efficient gene-delivery systems. This review will evaluate the problems and the potential solutions in this new field of medicine.
http://www.eurekaselect.com/106191/article
Gene delivery has attracted increasing interest as a highly promising therapeutic method to treat various diseases, including both genetic and acquired disorders. Viral-vectors based gene delivery can achieve higher transduction efficiency and long-term gene expression, but they may be associated with some shortcomings, such as immunogenicity ...
https://www.sciencedirect.com/science/article/pii/S1525001616327198
Hydrodynamic gene delivery has been shown to be able to promote both humoral- and cell-based immunity in animals in a gene dependent manner. Hydrodynamic gene delivery generates much more gene product than does the commonly used method of intramuscle injection of plasmid DNA.Author: Takeshi Suda, Dexi Liu
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