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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · Physical methods of nonviral gene delivery. Physical methods applied for in vitro and in vivo gene delivery are based on making transient penetration in cell membrane by mechanical, electrical, ultrasonic, hydrodynamic, or laser-based energy so that DNA entrance into the targeted cells is facilitated.
https://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/transfection-support/gene-delivery-selection-guide.html
Researchers have a wide variety of gene delivery techniques to introduce plasmid DNA, siRNA or duplex RNAi, oligonucleotides, and RNA into eukaryotic cells for a …
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/transfection-basics/gene-delivery-technologies.html
Viral delivery: Highest efficiency amongst gene delivery methods (80–90% transduction efficiency in primary cells) Works well with difficult to transfect cell types; Can be used for in vivo delivery of nucleic acids; Can be used for making stable cell lines (retroviral …
https://www.sciencedirect.com/science/article/pii/S0889858805700046
Direct DNA Delivery. Of the major methods of nonviral, mediated gene transfer, direct DNA delivery is probably the simplest, because it involves injection of a plasmid DNA containing a transgene into an organ site of choice.Author: Nelson A. Wivel, James M. Wilson
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722627/
Progress in molecular biology and especially, molecular medicine is now changing the basics of clinical medicine. A variety of viral and non-viral possibilities are available for basic and clinical research. This review summarizes the delivery routes and methods for gene transfer used in gene therapy.
https://www.sciencedirect.com/science/article/abs/pii/S0889858805700046
One of the most important characteristics is the ability of the virus to assure transport of its genomic DNA to the nucleus of the host cell without degradation by lysosomes. 45 It has become clear that one can take advantage of some of the barriers to gene expression by using viruses as the delivery vehicles, and this is discussed in more ...Author: Nelson A. Wivel, James M. Wilson
https://seekingalpha.com/article/4305556-gene-therapy-series-2-delivery-systems-in-gene-therapy-including-viral-vectors
Nov 11, 2019 · Examples of gene therapy methods using ex vivo delivery include CAR-T therapies like Yescarta and Kymriah. In vivo delivery: By definition, this means inside the body. In …
https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-018-1402-1
Feb 15, 2018 · Critical tissue defects frequently result from trauma, burns, chronic wounds and/or surgery. The ideal treatment for such tissue loss is autografting, but donor sites are often limited. Tissue engineering (TE) is an inspiring alternative for tissue repair and regeneration (TRR). One of the current state-of-the-art methods for TRR is gene therapy. Non-viral gene delivery systems (nVGDS) have ...Author: Pan Wu, Haojiao Chen, Ronghua Jin, Tingting Weng, Jon Kee Ho, Chuangang You, Liping Zhang, Xingang W...
http://www.eurekaselect.com/106191/article
In order to improve gene transfer efficacy, a lot of efforts have been made in the past years, and numerous gene carriers and techniques have been developed. In this review, we summarized the features, drawbacks and prospects of existing and emerging non-viral gene delivery methods.
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