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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3277219/
Nov 22, 2011 · An AAV variant with enhanced gene delivery to hESCs can serve as a valuable tool for numerous applications. For example, AAV could mediate the controlled overexpression of regulatory proteins, short hairpin RNAs, or microRNAs, which would enable basic investigations of the roles of key factors in stem cell self-renewal or lineage commitment, as ...Author: Prashanth Asuri, Prashanth Asuri, Melissa A Bartel, Melissa A Bartel, Tandis Vazin, Tandis Vazin, Ja...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2788045/
Aug 11, 2009 · After five rounds of selection, preliminary characterization of individual rAAV CMV-GFP generated with capsid clones from the AAV2 EP, AAV2 7mer, and chimeric AAV libraries failed to reveal variants with enhanced gene delivery (Supplementary Table S1, …Author: James T Koerber, Ryan Klimczak, Jae-Hyung Jang, Deniz Dalkara, John G Flannery, David V Schaffer
https://www.sciencedirect.com/science/article/pii/S0144861719310069
Reconstructed chitosan with alkylamine for enhanced gene delivery by promoting endosomal escape. Author links open overlay panel Guojun Huang a b Qi Chen a b Wangteng Wu a c Jianwei Wang a Paul K. Chu b Hongzhen Bai a Guping Tang a b. ... Gene delivery in vivo and tumor inhibition on A549 xenograft model.Author: Guojun Huang, Guojun Huang, Qi Chen, Qi Chen, Wangteng Wu, Jianwei Wang, Paul K. Chu, Hongzhen Bai, ...
https://pubs.acs.org/doi/10.1021/bc015575f
Overcoming the barriers to efficient gene transfer is a fundamental goal of biotechnology. A versatile approach to enhance the delivery of nonviral DNA involves complexation with cationic polymers, which can be designed to overcome the barriers to effective gene transfer. More recently, DNA release from a polymer substrate or scaffold has been shown to enhance gene transfer, likely by ...
https://www.sciencedirect.com/science/article/pii/S0928493116306634
Enhanced gene delivery of low molecular weight PEI by flower-like ZnO microparticles. ... the formed new complexes showed enhanced gene transfection compared to PEI/pDNA alone. Cell uptaking experiments explained a possible mechanism that the tips of ZnO microflowers penetrated into the surface of cells, thus facilitating the entry of gene ...Author: Ming Chen, Yaqin Tang, Tingting Wang, Qipeng Long, Ziying Zeng, Houwen Chen, Xuli Feng
https://www.nature.com/articles/3301141
Apr 12, 2000 · Electroporation was applied to enhance gene transfer into subcutaneous MC2 murine breast tumors. Cultured MC2 cells were also transfected …Author: J M Wells, L H Li, A Sen, G P Jahreis, S W Hui
https://www.nature.com/articles/nbt1182
Jan 22, 2006 · Surprisingly, both mutants also mediated enhanced gene delivery compared with wild type in the absence of serum . Analysis of mutations present …Author: Narendra Maheshri, James T Koerber, Brian K Kaspar, David V Schaffer
http://www.jbc.org/content/269/4/2550.short
Jan 28, 1994 · Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations. ... and data are accumulating which show their utility for in vivo gene transfer. ... The addition of 100 microM chloroquine in the transfection experiment enhanced the activity of the dioleyl compound by 4-fold and decreased the activity of the ...
https://pubs.acs.org/doi/abs/10.1021/nn101638u
Guanidinated amphiphilic cationic copolymer with enhanced gene delivery efficiency. Journal of Materials Chemistry 2012, 22 (36) , 18915. DOI: 10.1039/c2jm33141a. Yu Jin Kim, Sang-Mi Ryou, Sudeok Kim, Ji-Hyun Yeom, Min Su Han, Kangseok Lee, Maeng-Je Seong.Author: Shutao Guo, Yuanyu Huang, Qiao Jiang, Yun Sun, Liandong Deng, Zicai Liang, Quan Du, Jinfeng Xing, Yu...
https://dev.biologists.org/content/142/10/1885
May 15, 2015 · The variant mediated enhanced gene delivery to rat, mouse and human NSCs in vitro compared with several wild-type AAV serotypes (Jang et al., 2011). However, efficiency is distinct from selectivity, and in vitro is different from in vivo.Author: Melissa A. Kotterman, Melissa A. Kotterman, Tandis Vazin, Tandis Vazin, David V. Schaffer, David V. ...
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