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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3044603/
Sep 28, 2010 · Enhanced Gene Delivery and siRNA Silencing by Gold Nanoparticles Coated with Charge-Reversal Polyelectrolyte. ... The charge-reversal copolymers are usually used to improve the gene delivery efficiency by enhancing endosome ... It is known that localization into cytoplasm is the critical prerequisite of siRNA delivery to effectively silence ...Author: Shutao Guo, Yuanyu Huang, Qiao Jiang, Yun Sun, Liandong Deng, Zicai Liang, Quan Du, Jinfeng Xing, Yu...
https://pubs.acs.org/doi/abs/10.1021/nn101638u
Enhanced siRNA Delivery and Silencing Gold–Chitosan Nanosystem with Surface Charge-Reversal Polymer Assembly and Good Biocompatibility. ACS Nano 2012, 6 (8) , 7340-7351. DOI: 10.1021/nn3024688. Li-Chih Hu, Yusuke Yonamine, Shih-Hui Lee, …Author: Shutao Guo, Yuanyu Huang, Qiao Jiang, Yun Sun, Liandong Deng, Zicai Liang, Quan Du, Jinfeng Xing, Yu...
https://www.thermofisher.com/jp/en/home/references/ambion-tech-support/rnai-sirna/tech-notes/enhanced-sirna-delivery-and-long-term-gene-silencing.html
Figure 1. Gene Silencing After Retroviral Delivery of siRNA. Retroviral vectors expressing the indicated siRNA inserts from either the H1 or U6 promoter were transfected into a retrovirus packaging cell line in 6 well plates. Three days following infection, virus was collected and used to infect HeLa cells in triplicate.
https://www.pnas.org/content/115/12/E2696
Mar 20, 2018 · This demonstrates that the gene delivery vehicle itself can also modulate RNAi machinery activity, in addition to other mechanisms that have been previously exploited for enhanced gene silencing, such as the improvement of siRNA stability, uptake, and endosomal escape.Author: Jiahe Li, Connie Wu, Wade Wang, Yanpu He, Elad Elkayam, Elad Elkayam, Leemor Joshua-Tor, Leemor Josh...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4391875/
Enhanced Gene Silencing through Human Serum Albumin-Mediated Delivery of Polyethylenimine-siRNA Polyplexes. ... Most notable is the enhanced delivery and efficacy of paclitaxel when delivered as an albumin conjugated nanoparticle, Abraxane .Author: Elena Nicolì, Elena Nicolì, Marie Isabel Syga, Michela Bosetti, V. Prasad Shastri
https://pubs.acs.org/doi/full/10.1021/nn101638u
Charge-reversal functional gold nanoparticles first prepared by layer-by-layer technique were employed to deliver small interfering RNA (siRNA) and plasmid DNA into cancer cells. Polyacrylamide gel electrophoresis measurements of siRNA confirmed the occurrence of the charge-reversal property of functional gold nanoparticles. The expression efficiency of enhanced green fluorescent protein (EGFP ...Author: Shutao Guo, Yuanyu Huang, Qiao Jiang, Yun Sun, Liandong Deng, Zicai Liang, Quan Du, Jinfeng Xing, Yu...
https://academic.oup.com/nar/article/48/1/75/5644999
Nov 28, 2019 · Lidya Salim, Golam Islam, Jean-Paul Desaulniers, Targeted delivery and enhanced gene-silencing activity of centrally modified folic acid–siRNA conjugates, Nucleic Acids Research, Volume 48, Issue 1, ... Click-modified anandamide siRNA enables delivery and …Author: Lidya Salim, Golam Islam, Jean-Paul Desaulniers
https://www.pnas.org/content/pnas/115/12/E2696.full.pdf
To demonstrate that enhanced gene silencing can be achieved by delivery of siRNA/Ago2 complexes, we first used commercial polymer or lipid-based transfection reagents designed for in vitro use to examine the impact of preassembly of the complex versus siRNA alone. We then synthesized a …Author: Jiahe Li, Connie Wu, Wade Wang, Yanpu He, Elad Elkayam, Elad Elkayam, Leemor Joshua-Tor, Leemor Josh...
https://www.thermofisher.com/jp/ja/home/references/ambion-tech-support/rnai-sirna/tech-notes/enhanced-sirna-delivery-and-long-term-gene-silencing.html
Figure 1. Gene Silencing After Retroviral Delivery of siRNA. Retroviral vectors expressing the indicated siRNA inserts from either the H1 or U6 promoter were transfected into a retrovirus packaging cell line in 6 well plates. Three days following infection, virus was collected and used to infect HeLa cells in triplicate.
https://briefs.techconnect.org/wp-content/volumes/Nanotech2008v2/pdf/1381.pdf
siRNA into cells: the delivery by transfection of synthetic siRNA duplexes into the cytoplasm and the in situ delivery of siRNAs expressed from a plasmid (or DNA cassettes) preliminary introduced by gene transfer into the nucleus. In this report, we present many delivery systems enhancing siRNA delivery. 2. siRNA DELIVERY WITH NON-VIRAL VECTORSAuthor: P. Neuberg, J-P . Behr, P. Erbacher, Boulevard Sébastien Brandt
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