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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2694965/
In contrast, the PTD-DRBD siRNA delivery approach described here fulfills many of the criteria for an efficient siRNA delivery system into primary cells. PTD-DRBD delivered siRNAs and induced RNAi responses in the entire population of three difficult to deliver primary cell types (T cells, HUVECs, and hESCs) in a rapid and non-cytotoxic fashion.Author: Akiko Eguchi, Akiko Eguchi, Akiko Eguchi, Bryan R Meade, Bryan R Meade, Yung-Chi Chang, Craig T Fred...
http://www.nature.com/articles/nbt.1541
May 17, 2009 · Thus, PTD-DRBD–mediated siRNA delivery allows efficient gene silencing in difficult-to-transfect primary cell types. The delivery to primary cells is a major challenge in the application of ...Author: Akiko Eguchi, Akiko Eguchi, Akiko Eguchi, Bryan R Meade, Bryan R Meade, Yung-Chi Chang, Craig T Fred...
https://www.cirm.ca.gov/about-cirm/publications/efficient-sirna-delivery-primary-cells-peptide-transduction-domain-dsrna
Efficient siRNA delivery into primary cells by a peptide transduction domain-dsRNA binding domain fusion protein.
https://www.pancreapedia.org/tools/methods/highly-efficient-sirna-delivery-into-primary-cultures-of-rat-pancreatic-stellate-cells
Delivery of siRNA into PSCs provides a valuable tool for selectively silencing genes to examine their cellular function. Several factors can influence the degree to which a target gene is silenced in vitro including: 1) transfection efficiency; 2) transcription rate of the target gene; 3) protein stability and turnover; 4) efficacy of the siRNA sequence; and 5) growth characteristics of the cells.Author: Phoebe A Phillips
https://www.cirm.ca.gov/printpdf/about-cirm/publications/efficient-sirna-delivery-primary-cells-peptide-transduction-domain-dsrna
RNA interference (RNAi) induced by short interfering RNA (siRNA) allows for discovery research and large-scale screening; however, owing to their size and anionic charge, siRNAs do not readily enter cells. Current approaches do not deliver siRNAs into a high. percentage of primary cells …
https://www.researchgate.net/publication/24433508_Efficient_siRNA_Delivery_into_Primary_Cells_by_Peptide_Transduction-dsRNA_Binding_Domain_PTD-DRBD_Fusion_Protein
Efficient siRNA Delivery into Primary Cells by Peptide Transduction-dsRNA Binding Domain (PTD-DRBD) Fusion Protein Article (PDF Available) in Nature Biotechnology 27(6):567-71 · June 2009 with ...
https://www.patentdocs.org/2009/08/efficient-delivery-of-sirna.html
By Kevin E. Noonan -- A research paper in the June 2009 edition of Nature Biotechnology (Eguchi et al., 2009, "Efficient siRNA delivery into primary cells by a peptide transduction domain–dsRNA binding domain fusion protein," Nature Biotechnology 27: 567-71) describes a new system for introducing short interfering RNAs (siRNAs) into mammalian cells that may help this technology become practicable …
https://www.nature.com/articles/nbt.3078
Nov 17, 2014 · Current siRNA delivery has begun to move away from large lipid and synthetic nanoparticles to more defined molecular conjugates 9. Here we address this issue by synthesis of short interfering ribonucleic neutrals (siRNNs) whose phosphate backbone contains neutral phosphotriester groups, allowing for delivery into cells.Author: Bryan R Meade, Khirud Gogoi, Alexander S Hamil, Caroline Palm-Apergi, Arjen van den Berg, Jonathan C...
https://onlinelibrary.wiley.com/doi/full/10.1002/wnan.1634
Polyplexes made from siRNA complexed with Tf‐PEI and Mel‐PEI are shown to be specifically taken up by ATCs facilitating endosomal release necessary for efficient gene silencing. By spray drying, dry ...
https://www.polyplus-transfection.com/products/pulsin/
The delivery of protein and antibody using PULSin represents a powerful approach for functional studies.For example, PULSin enables you to study lethal proteins by controlling the level and time course of protein delivery into the cells.Cell types: Adherent & suspension cells grown in presence of serum
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