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http://www.biopharminternational.com/dna-vaccine-delivery
Oct 02, 2011 · A DNA vaccine is a plasmid produced in Escherichia coli that contains an antigen gene controlled by a promoter that functions only in animal cells, usually the cytomegalovirus promoter. The DNA vaccine plasmid must be delivered to antigen-presenting cells (APCs) of the host, where the antigenic protein is expressed, processed, and presented to the immune system.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274812/
Nov 15, 2018 · More recently, the DNA vaccine delivery properties of orally applied bacteria, for example with regard to phagolysosomal escape have been improved by additional coating with NC . Compared with other sytemic routes of DNA vaccination, pulmonary application of aerolized DNA vaccines is a rather new approach .Author: Dominika Hobernik, Matthias Bros
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465129/
Viral and nonviral methods of DNA vaccine delivery. Viral vectors such as retrovirus, adeno virus, herpes simplex virus, vaccinia virus are efficient in DNA transfer due to their nanoscale dimensions, well characterized surface properties allowing the incorporation …
https://link.springer.com/article/10.1007%2FBF03402065
DNA vaccines have been delivered to a wide variety of tissues by both injected and noninjected DNA delivery methods, including (i) into the muscle by gene gun (GG) or intramuscular (IM) injection (13,15–20); (ii) into the skin by epidermal GG delivery (9,12,21) or intradermal (ID) (12,22,23) or subcutaneous (SC) (12,24) injection; (iii) into the circulatory system by intravenous (IV) injection …Author: Michael J. McCluskie, Michael J. McCluskie, Cynthia L. Brazolot Millan, Robert A. Gramzinski, Harrie...
https://phys.org/news/2016-12-nanoparticle-based-method-dna-vaccine-delivery.html
Dec 19, 2016 · Nanoparticle-based method shows promise in DNA vaccine delivery by Brigham and Women's Hospital Scientists at Brigham and Women's Hospital have developed a novel method for delivering therapeutic...
https://www.hindawi.com/journals/jir/2019/8303648/
Intradermal immunization for vaccine delivery is an upcoming strategy showing significant advantages over conventional vaccination routes. In particular, in the last few years, intradermal vaccination has gained momentum as an alternative and more effective vaccine delivery route, both from a scientific and a commercial point of view (Table 3).Author: E. Criscuolo, V. Caputo, V. Caputo, R. A. Diotti, R. A. Diotti, G. A. Sautto, G. A. Kirchenbaum, N. ...
https://www.researchgate.net/publication/327803093_DNA_vaccine_Methods_and_mechanisms
Since DNA vaccines are easily designed and manufactured, they are easier to preserve them, and they are inexpensive, as one of the most desirable types of vaccine.
https://wwwnc.cdc.gov/eid/article/2/3/96-0302_article
A novel and powerful method for vaccine research, colloquially known as DNA vaccines, involves the deliberate introduction into tissues of a DNA plasmid carrying an antigen-coding gene that transfects cells in vivo and results in an immune response.
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