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https://www.nature.com/articles/nbt0699_551
Sustained delivery of plasmid DNA from the polymer matrix may transfect large numbers of cells at a localized site leading to production of a therapeutic protein to enhance tissue development.Author: Lonnie D. Shea, Elizabeth Smiley, Jeffrey Bonadio, David J. Mooney
https://www.sciencedirect.com/science/article/pii/S1461534700003059
Nov 01, 2000 · Delivering DNA with polymer matrices: applications in tissue engineering and gene therapy. ... The first use of polymers to deliver DNA in tissue engineering applications 9 involved the delivery of plasmids encoding for BMPs and fragments of human parathyroid hormone genes. Bone tissue is an appropriate target for this approach owing to its ...Author: Sara-Kaye Madsen, David J Mooney
https://mooneylab.seas.harvard.edu/publications/dna-delivery-polymer-matrices-tissue-engineering
Shea LD, Smiley E, Bonadio J, Mooney DJ. DNA delivery from polymer matrices for tissue engineering. Nat Biotechnol. 1999;17 (6) :551-4.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1949490/
May 30, 2007 · 4.1 Scaffold Encapsulation. Many materials used for controlled drug delivery are also used to fabricate tissue engineering scaffolds. Release from the scaffolds occurs by a combination of polymer degradation and vector diffusion (Figure 1).A critical aspect associated with the encapsulation of gene therapy vectors is that the scaffold fabrication method must be compatible with the vector ...Author: Laura De Laporte, Lonnie D. Shea
https://www.sciencedirect.com/science/article/abs/pii/S1461534700003059
Delivering DNA with polymer matrices: applications in tissue engineering and gene therapy Author links open overlay panel Sara-Kaye Madsen a David J Mooney b Show moreAuthor: Sara-Kaye Madsen, David J Mooney
https://www.hindawi.com/journals/ijps/2011/290602/
Scaffolds have been used for tissue engineering such as bone, cartilage, ligament, skin, vascular tissues, neural tissues, and skeletal muscle and as vehicle for the controlled delivery of drugs, proteins, and DNA.Author: Brahatheeswaran Dhandayuthapani, Yasuhiko Yoshida, Toru Maekawa, D. Sakthi Kumar
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2648403/
Efficient and controlled gene delivery from biomaterials has the potential to enhance the applicability of gene transfer in tissue engineering. In this report, a substrate-mediated strategy was used to deliver DNA complexes from hyaluronic acid-collagen hydrogels.Author: Tatiana Segura, Peter H. Chung, Lonnie D. Shea
https://www.researchgate.net/publication/6319154_Matrices_and_Scaffolds_for_DNA_Delivery_in_Tissue_Engineering
Matrices and Scaffolds for DNA Delivery in Tissue Engineering Article · Literature Review in Advanced Drug Delivery Reviews 59(4-5):292-307 · June 2007 with 86 Reads How we measure 'reads'
https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(05)00181-4
Tissue engineering scaffolds capable of sustained plasmid release can promote gene transfer locally and stimulate new tissue formation. We have investigated the scaffold design parameters that influence the extent and duration of transgene expression and have characterized the distribution of transfected cells.Author: Jae Hyung Jang, Christopher B. Rives, Lonnie D. Shea
https://www.researchgate.net/publication/8970955_Gene_Delivery_in_Tissue_Engineering_A_Photopolymer_Platform_to_Coencapsulate_Cells_and_Plasmid_DNA
Gene Delivery in Tissue Engineering: A Photopolymer Platform to Coencapsulate Cells and Plasmid DNA. ... many benefits for designing DNA delivery matrices for tissue.
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