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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · Physical methods of nonviral gene delivery. Physical methods applied for in vitro and in vivo gene delivery are based on making transient penetration in cell membrane by mechanical, electrical, ultrasonic, hydrodynamic, or laser-based energy so that DNA entrance into the targeted cells is facilitated.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722627/
Progress in molecular biology and especially, molecular medicine is now changing the basics of clinical medicine. A variety of viral and non-viral possibilities are available for basic and clinical research. This review summarizes the delivery routes and methods for gene transfer used in gene therapy.
https://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/transfection-support/gene-delivery-selection-guide.html
Researchers have a wide variety of gene delivery techniques to introduce plasmid DNA, siRNA or duplex RNAi, oligonucleotides, and RNA into eukaryotic cells for a …
https://www.sciencedirect.com/science/article/pii/S0889858805700046
Direct DNA Delivery. Of the major methods of nonviral, mediated gene transfer, direct DNA delivery is probably the simplest, because it involves injection of a plasmid DNA containing a transgene into an organ site of choice.Author: Nelson A. Wivel, James M. Wilson
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/transfection-basics/gene-delivery-technologies.html
Gene Delivery Technologies ... researchers have developed a number of techniques each using a different approach—from using chemicals and carrier molecules that coat the nucleic acids to neutralize them to physical methods that create transient pores in the membrane to introduce the DNA directly into the cell. ... Highest efficiency amongst ...
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