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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6859480/
SIGS vs HIGS: a study on the efficacy of two dsRNA delivery strategies to silence Fusarium FgCYP51 genes in infected host and non‐host plants. Aline Koch, 1 , † Lisa Höfle, 1 , † Bernhard Timo Werner, 1 Jafargholi Imani, 1 Alexandra Schmidt, 1 Lukas Jelonek, 2 and Karl‐Heinz Kogel 1Author: Aline Koch, Lisa Höfle, Bernhard Timo Werner, Jafargholi Imani, Alexandra Schmidt, Lukas Jelonek, Ka...
https://www.ncbi.nlm.nih.gov/pubmed/29407675
Mar 10, 2018 · Tribolium castaneum was effectively killed using a combination of BiP-dsRNA and Armet-dsRNA complexed with BAPCs; most dying as larvae or during eclosion (~75%). Feeding dsRNA alone resulted in fewer deaths (~30%). The results show that complexation of dsRNA with BAPCs enhanced the oral delivery of dsRNA over dsRNA alone.Author: L.A. Avila, L.A. Avila, R. Chandrasekar, K.E. Wilkinson, J. Balthazor, J. Balthazor, M. Heerman, J. ...
http://onlinelibrary.wiley.com/doi/10.1111/j.1744-7917.2012.01534.x/abstract
This review focuses on the approaches of introducing dsRNA into insect cells or insect bodies to induce effective RNAi. The three most common delivery methods, namely, microinjection, ingestion, and soaking, are illustrated in details and their advantages and limitations are summarized for purpose of feasible RNAi research.
https://www.sciencedirect.com/science/article/pii/S2095311913605110
However, one of the key limitations in pest control is lack of the convenient and efficient method for dsRNA delivery. In this study, different dsRNA delivery methods at their own optimum conditions were evaluated comparatively for their efficiency with Helicoverpa armigera as test animal. It was found that the popular one-time injection of ...Author: Jing Yang, Zhao-jun Han
https://onlinelibrary.wiley.com/doi/full/10.1002/ps.5291
Contributions of dsRNases to differential RNAi efficiencies between the injection and oral delivery of dsRNA in Locusta migratoria. Huifang Song. Research Institute of Applied Biology, Shanxi University, Taiyuan, China. College of Life Science, Shanxi University, Taiyuan, China.Author: Huifang Song, Yunhe Fan, Jianqin Zhang, Anastasia Mw Cooper, Kristopher Silver, Daqi Li, Tao Li, Enb...
https://www.jove.com/video/57390/double-stranded-rna-oral-delivery-methods-to-induce-rna-interference
Clay soil amendment delivery of dsRNA into plants through soil. A new line of delivering material: clay that is a dust free nucleic acid carrier for use in delivering active ingredients such as dsRNA to soils for uptake in plants and ultimately into insects. (A) Unbaked clay and (B) baked clay depicting water tolerance/retention.Author: Saikat Kumar B. Ghosh, Wayne B. Hunter, Alexis L. Park, Dawn E. Gundersen-Rindal
https://www.frontiersin.org/articles/10.3389/fphys.2017.00256/full
Apr 28, 2017 · The strategy of oral dsRNA delivery involves the exposure of dsRNA to the gut lumen environment. Here, one crucial obstacle is the presence of gut nucleases. We speculated that the interaction of PTD-DRBD with dsRNA might offer protection against nuclease activity by covering the dsRNA.Author: François-Xavier Gillet, Rayssa A. Garcia, Leonardo L. P. Macedo, Erika V. S. Albuquerque, Maria C. M...
https://www.researchgate.net/profile/Na_Yu5/publication/255976198_Delivery_of_dsRNA_for_RNAi_in_insects_An_overview_and_future_directions/links/5c67685e299bf1e3a5abe9f5/Delivery-of-dsRNA-for-RNAi-in-insects-An-overview-and-future-directions.pdf
Insect Science (2013) 20, 4–14, DOI 10.1111/j.1744-7917.2012.01534.x REVIEW Delivery of dsRNA for RNAi in insects: an overview and future directions Na Yu †, Olivier Christiaens , Jisheng Liu ...
https://academic.oup.com/jee/article/112/1/434/5227427
Dec 03, 2018 · While injection of the dsRNA directly into the target insect hemocoel is likely the most efficient dsRNA delivery method to investigate phenotypic effects, it is not practical for the development of an RNAi-based control method. Oral administration of dsRNA (ingestion) would be more feasible; however, the target dsRNA must survive in the midgut ...Author: Man-Yeon Choi, Robert K Vander Meer
https://www.sciencedirect.com/science/article/pii/S0048357519304596
Oct 22, 2019 · Successful delivery of dsRNA depends on the capacity of NPs to enter the cell, reach the cytoplasm, and then release the cargo . In most cases, NPs are internalized by an endocytosis and endo-lysosomal pathway. Therefore, endosomal escape is of particular importance for the delivery of dsRNA …Author: Kangxu Wang, Kangxu Wang, Yingchuan Peng, Jiasheng Jason Chen, Yue Peng, Xuesong Wang, Zihan Shen, Z...
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