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https://www.ncbi.nlm.nih.gov/pubmed/23955821
This review focuses on the approaches of introducing dsRNA into insect cells or insect bodies to induce effective RNAi. The three most common delivery methods, namely, microinjection, ingestion, and soaking, are illustrated in details and their advantages and limitations are summarized for purpose of feasible RNAi research.Author: Na Yu, Olivier Christiaens, Jisheng Liu, Jinzhi Niu, Kaat Cappelle, Silvia Caccia, Hanneke Huvenne, ...
https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1744-7917.2012.01534.x
This review focuses on the approaches of introducing dsRNA into insect cells or insect bodies to induce effective RNAi. The three most common delivery methods, namely, microinjection, ingestion, and soaking, are illustrated in details and their advantages and limitations are summarized for purpose of feasible RNAi research.Author: Na Yu, Olivier Christiaens, Jisheng Liu, Jinzhi Niu, Kaat Cappelle, Silvia Caccia, Hanneke Huvenne, ...
https://www.researchgate.net/publication/255976198_Delivery_of_dsRNA_for_RNAi_in_insects_An_overview_and_future_directions
Soaking involves literally soaking the insect or cells in a solution of dsRNA, and is a common method of RNAi delivery in insect cell lines rather than the organism itself (Yu et al., 2012). ...
https://www.researchgate.net/profile/Na_Yu5/publication/255976198_Delivery_of_dsRNA_for_RNAi_in_insects_An_overview_and_future_directions/links/5c67685e299bf1e3a5abe9f5/Delivery-of-dsRNA-for-RNAi-in-insects-An-overview-and-future-directions.pdf
dsRNA delivery to insects and its use in pest control. Delivery of dsRNAs through Injection Microinjection was already used by Fire and Mello in the early days of RNAi …
https://www.frontiersin.org/articles/10.3389/fphys.2018.01912/full
Jan 11, 2019 · The bacterial system, in its simplicity, is one of the most successful methods of dsRNA delivery in insects. This system makes use of the genetically modified HT115 bacterial strain, which lacks the dsRNA-degrading bacterial endonuclease RNase III. Furthermore, this strain contains the T7 polymerase gene, controlled by the inducible lac operon.Author: Elise Vogel, Dulce Santos, Lina Mingels, Thomas-Wolf Verdonckt, Jozef Vanden Broeck
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0171861
RNAi technology may serve as a viable tool to manage this voracious pest, but delivery of dsRNA to piercing-sucking insects has posed a tremendous challenge. Effective and practical use of RNAi as molecular biopesticides for biocontrol of insects like BMSB in the environment requires that dsRNAs be delivered in vivo through ingestion.Author: Saikat Kumar B. Ghosh, Wayne B. Hunter, Alexis L. Park, Dawn E. Gundersen-Rindal
https://www.jove.com/video/57390/double-stranded-rna-oral-delivery-methods-to-induce-rna-interference
Various methods that have been used for dsRNA delivery to insects and insect cells to induce systemic RNAi include feeding 10, 19, soaking 20, 21, microinjection 22, carriers such as liposomes 23, and other techniques 24.Author: Saikat Kumar B. Ghosh, Wayne B. Hunter, Alexis L. Park, Dawn E. Gundersen-Rindal
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112363/
Nov 17, 2016 · Delivery of dsRNA using chemically modified molecules, polymer nanoparticles, liposomes, viruses or bacteria, could increase efficacy in attaining a potent RNAi response. The choice of the delivery method and the choice of formulation would of course depend on the circumstances, on the target insect and on the reason for impaired RNAi-efficiency.Author: Mallikarjuna R. Joga, Moises J. Zotti, Guy Smagghe, Olivier Christiaens
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