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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3109217/
Electroporation Enhanced DNA Vaccine Delivery. Electroporation (EP) is a method to introduce macromolecules such as nucleic acids into cells, either in vivo or in vitro, via the application of brief electric pulses to induce transient and reversible permeabilization of the cell membrane (Figure 1).Author: Niranjan Y Sardesai, David B Weiner
https://link.springer.com/protocol/10.1385/1-59745-168-1:73
This is especially true in species other than mice. One way of improving this efficiency is to temporarily permeabilize the cells to allow cellular uptake of DNA plasmids. One way to permeabilize cells is by electroporation. The current report describes some of the parameters for optimizing electroporation for enhancing the level of gene expression.Author: Shawn Babiuk, Sylvia van Drunen Littel-van den Hurk, Lorne A. Babiuk
https://www.ncbi.nlm.nih.gov/pubmed/16988447
Delivery of DNA vaccines using electroporation. Babiuk S(1), van Drunen Littel-van den Hurk S, Babiuk LA. Author information: (1)Canadian Food Inspection Agency, Government of Canada, Winnipeg, Manitoba, Canada.Author: Shawn Babiuk, Sylvia van Drunen Littel-van den Hurk, Lorne A. Babiuk
https://www.researchgate.net/publication/6806882_Delivery_of_DNA_Vaccines_Using_Electroporation
Delivery of DNA Vaccines Using Electroporation Article · Literature Review (PDF Available) in Methods in molecular medicine 127:73-82 · February 2006 with 104 Reads How we measure 'reads'
https://www.academia.edu/31057413/Delivery_of_DNA_Vaccines_Using_Electroporation
Delivery of DNA Vaccines Using Electroporation. DNA Vaccines, 2006. S. Babiuk. Download with Google Download with Facebook or download with email. Delivery of DNA Vaccines Using Electroporation. Download. Delivery of DNA Vaccines Using Electroporation.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2835112/
Mar 31, 2009 · Electroporation (EP) has been used in basic research for the past 25 years to aid in the transfer of DNA into cells in vitro. EP in vivo enhances transfer of DNA vaccines and therapeutic plasmids to the skin, muscle, tumors, and other tissues resulting in high levels of expression, often with serological and clinical benefits.Author: Angela M Bodles-Brakhop, Richard Heller, Ruxandra Draghia-Akli
https://www.sciencedirect.com/science/article/pii/S0952791511000331
Electroporation enhanced DNA vaccine delivery Electroporation (EP) is a method to introduce macromolecules such as nucleic acids into cells, either in vivo or in vitro, via the application of brief electric pulses to induce transient and reversible permeabilization of the cell membrane (Figure 1).Author: Niranjan Y Sardesai, David B Weiner
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4635908/
May 18, 2015 · In this review, the use of electroporation as a DNA delivery method, the strategies used to enhance the immune responses, the cancer antigens that have been tested, and the escape mechanism (s) used by tumor cells are discussed, with a focus on the progress of clinical trials using cancer DNA vaccines.Author: Si-Hyeong Lee, Sayyed Nilofar Danishmalik, Jeong-Im Sin
https://www.nature.com/articles/s41598-017-04547-2
Jun 23, 2017 · In vivo electroporation (EP) is used to enhance the uptake of nucleic acids and its association with DNA vaccination greatly stimulates immune responses to vaccine antigens delivered …Author: Biliana Todorova, Lucille Adam, Slobodan Culina, Raphaël Boisgard, Frédéric Martinon, Antonio Cosma,...
https://www.intechopen.com/books/application-of-nanotechnology-in-drug-delivery/electroporation-advantages-and-drawbacks-for-delivery-of-drug-gene-and-vaccine
Electroporation-based DNA delivery technology dramatically enhances cellular uptake of DNA vaccines [Fioretti et al., 2014]. Preliminary data from an ongoing clinical trial showed electroporation enhanced the frequency and the magnitude of the anti-HIV-1 T-cell response [ …Author: Azam Bolhassani, Afshin Khavari, Zahra Orafa
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