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https://en.wikipedia.org/wiki/Cell-penetrating_peptide
Cell-penetrating peptides (CPP) are able to transport different types of cargo molecules across plasma membrane; thus, they act as molecular delivery vehicles. They have numerous applications in medicine as drug delivery agents in the treatment of different diseases including cancer and virus inhibitors, as well as contrast agents for cell labeling.
https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/iub.1257
Mar 23, 2014 · During the last 2 decades, CPP have been widely studied as delivery vectors 16 - 18 and have been increasingly used to transport small RNA/DNA, plasmids, antibodies, and …Author: Inês Rego de Figueiredo, João Miguel Freire, Luís Flores, Ana Salomé Veiga, Miguel A.R.B. Castanho
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032848/
Dec 17, 2013 · Plasmid-mediated delivery of these components into cells can result in uncontrolled integration of the plasmid sequence into the host genome, and unwanted immune responses and potential safety problems that can be caused by the bacterial sequences.Author: Suresh Ramakrishna, Abu-Bonsrah Kwaku Dad, Jagadish Beloor, Ramu Gopalappa, Sang-Kyung Lee, Hyongbum...
https://www.hindawi.com/journals/bmri/2015/834079/
Most studies have focused on CPP-mediated delivery into mammalian cells, although our research team has demonstrated that CPPs can deliver biologically active molecules into a variety of species, including rotifers , cyanobacteria , insects , plants , and paramecium .Author: Yue-Wern Huang, Han-Jung Lee, Larry M. Tolliver, Robert S. Aronstam
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635728/
Principles of peptide-based nucleic acid delivery systems. ... To date there are many examples of CPP-mediated delivery of plasmid DNA into cultured cells and also in vivo. Due to the size of plasmids and the resulting high number of negative charges, only a non-covalent approach has proved feasible.Author: Sandra Veldhoen, Sandra D. Laufer, Tobias Restle
https://www.nature.com/articles/s41598-017-17316-y
Dec 06, 2017 · We have previously shown that a CPP, PF14 6 is an efficient plasmid DNA delivery vector in both cell cultures 7 and in mouse models after systemic administration 4. PF14 is …Author: Kaido Kurrikoff, Kadi-Liis Veiman, Kadri Künnapuu, Elin Madli Peets, Tõnis Lehto, Ly Pärnaste, Piret...
https://www.pnas.org/content/115/19/4903
May 08, 2018 · We hypothesize that using CPP alone as the delivery vector may be even more potent; a single-component delivery vector is also advantageous for eventual translation. However, for Cas9 expression plasmid delivery, the conventional CPPs either are too small or lack an adequate cationic charge density to function as stand-alone gene vectors.Author: Hong Xia Wang, Ziyuan Song, Yeh Hsing Lao, Xin Xu, Jing Gong, Du Cheng, Syandan Chakraborty, Ji Sun ...
https://www.cell.com/molecular-therapy-family/molecular-therapy/pdf/S1525-0016(16)32515-1.pdf
Plasmid delivery with unmodified CPPs has been described in a handful of papers, but transfection efficiencies have generally been relatively poor in most of these reports.13–15 To corroborate this, Chinese hamster ovary (CHO) cells were transfected with a luciferase-encoding plasmid complexed with either TP10 or Arg9.
https://www.nature.com/articles/s41598-019-42456-8
Apr 18, 2019 · Here we use green fluorescent protein (GFP) as a model cargo to compare delivery capacity of five CPP sequences (Penetratin, R8, TAT, Transportan, Xentry) and cyclic derivatives in different human ...Author: Sanjay G. Patel, Edward J. Sayers, Lin He, Rohan Narayan, Thomas L. Williams, Emily M. Mills, Rudolf...
https://www.sciencedirect.com/science/article/pii/S016836591830395X
Sep 10, 2018 · FGF2B (TYRSRKYTSWYVALKR) is a short 16-residue peptide that shows no significant cell penetrating properties by itself, however when coupled to a CPP it demonstrated up to two orders of magnitude enhanced delivery of recombinant proteins into a wide variety of cell types compared to an unmodified CPP . Herein we describe GET transfection ...Author: Gizem Osman, Jason Rodriguez, Sze Yan Chan, Jane Chisholm, Jane Chisholm, Gregg Duncan, Gregg Duncan...
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