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https://www.sciencedirect.com/science/article/pii/S152500160200031X
In this study, we used commercially available cationic liposomes (LipofectAMINE) and polymers (Superfect) to assess the feasibility of non-viral gene delivery to human pancreatic islets. We used green fluorescent protein expression plasmid (pCMS-EGFP) as a reporter gene and human vascular endothelial growth factor expression plasmid (pCAGGS-hVEGF) as a therapeutic gene.Author: Ram I Mahato, James Henry, Ajit S Narang, Omaima Sabek, Daniel Fraga, Malak Kotb, A.Osama Gaber
https://www.ncbi.nlm.nih.gov/pubmed/12573622
Cationic lipid and polymer-based gene delivery to human pancreatic islets. Mahato RI(1), Henry J, Narang AS, Sabek O, Fraga D, Kotb M, Gaber AO. Author information: Transplantation of pancreatic islets has great potential for treating Type I diabetes.Author: Ram I Mahato, James Henry, Ajit S Narang, Omaima Sabek, Daniel Fraga, Malak Kotb, A.Osama Gaber
https://www.cell.com/molecular-therapy-family/molecular-therapy/comments/S1525-0016(02)00031-X
Jan 01, 2003 · Cationic lipid and polymer-based gene delivery to human pancreatic islets Previous Article Free cationic liposomes inhibit the inflammatory response to cationic lipid–DNA complex injected intravenously and enhance its transfection efficiencyAuthor: Ram I Mahato, James Henry, Ajit S Narang, Omaima Sabek, Daniel Fraga, Malak Kotb, A.Osama Gaber
https://nebraska.pure.elsevier.com/en/publications/cationic-lipid-and-polymer-based-gene-delivery-to-human-pacreatic
Ex vivo gene therapy may promote re-vascularization or inhibit apoptosis of the islets and promote graft. In this study, we investigated the feasibility of non-viral gene delivery using Enhanced Green Fluorescent Protein (EGFP) and human Vascular Endothelial Growth Factor (hVEGF 165 ) expression plasmids as model reporter and therapeutic genes.Author: Ram I Mahato, James Henry, Ajit S Narang, Omaima Sabek, Daniel Fraga, Malak Kotb, A.Osama Gaber
https://www.researchgate.net/publication/10911521_Cationic_Lipid_and_Polymer-based_Gene_Delivery_to_Human_Pancreatic_Islets
Cationic Lipid and Polymer-based Gene Delivery to Human Pancreatic Islets Article (PDF Available) in Molecular Therapy 7(1):89-100 · February 2003 with 110 Reads How we measure 'reads'
https://academic.oup.com/abbs/article/46/2/141/1569
Dec 29, 2013 · Cationic lipid and polymer-based gene delivery to human pancreatic islets, ... S, Chroboczek. J. Standardization of procedure for efficient ex vivo gene transfer into porcine pancreatic islets with cationic liposomes, ... Transfection efficiency and cytotoxicity of nonviral gene transfer reagents in human smooth muscle and endothelial cells, ...
https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-10-28
Mar 30, 2010 · Modulation of gene expression in the context of intact pancreatic islets is particularly difficult because of their three dimensional structure (50-500 μm in diameter), making physical access to the islet core difficult. Islets are organized in organoid …Author: Bruno Lefebvre, Bruno Lefebvre, Brigitte Vandewalle, Brigitte Vandewalle, Justine Longue, Justine Lo...
https://www.sciencedirect.com/science/article/pii/S0168365918301202
The clinical success of chimeric antigen receptor (CAR) T cell immunotherapy in treating multiple blood cancers has created a need for efficient methods of ex vivo gene delivery to primary human T cells for cell engineering. Here, we synthesize and evaluate a panel of cationic polymers for gene delivery to both cultured and primary human T cells.Author: Brynn R. Olden, Yilong Cheng, Jonathan L. Yu, Suzie H. Pun
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