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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395777/
Feb 03, 2015 · The DNA transposon piggyBac is a potential therapeutic agent for multiple genetic diseases such as cystic fibrosis (CF). Recombinant piggyBac transposon and transposase are typically codelivered by plasmid transfection; however, plasmid delivery is inefficient in somatic cells in vivo and is a barrier to the therapeutic application of transposon-based vector systems.Author: Ashley L Cooney, Brajesh K Singh, Patrick L Sinn
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175031/
Two new transposon delivery vector systems utilizing Mariner and mini-Tn10 transposons have been developed for in vivo insertional mutagenesis in Bacillus anthracis and other compatible Gram-positive species. The utility of both systems was directly demonstrated through the mutagenesis of a widely used B. anthracis strain.Author: Adam C. Wilson, Marta Perego, James A. Hoch
https://msystems.asm.org/content/3/1/e00143-17
Feb 27, 2018 · (A to E) The five part vectors that are used for Golden Gate assembly of the magic pool transposon delivery vectors: part1 vector, part2 vector, part3 vector, barcoded part4 vector, and part5 vector (not drawn to scale). We show the sequences of the 4-nucleotide overhangs for Golden Gate assembly. (F) The transposon delivery vector with DNA ...Author: Hualan Liu, Morgan N. Price, Robert Jordan Waters, Jayashree Ray, Hans K. Carlson, Jacob S. Lamson, ...
https://www.systembio.com/wp-content/uploads/Manual_PiggyBac_Web.pdf
0.5 g PB Cumate switch Transposon vector clone (PBQM812A-1) + 0.2 g PiggyBac Transposase vector (PB210PA-1) 8.0 l SBI’s PureFection transfection reagent 50 μl of serum-free DMEM 2. After transfection of cells, split the cells into multiple wells of a 6-well plate (or a …
https://www.osti.gov/pages/biblio/1434009-magic-pools-parallel-assessment-transposon-delivery-vectors-bacteria
To meet this challenge, we describe a part-based strategy for constructing libraries of hundreds of transposon delivery vectors, which we term “magic pools.” Within a magic pool, each transposon vector has a different combination of upstream sequences (promoters and ribosome binding sites) and antibiotic resistance markers as well as a ...Author: Hualan Liu, Morgan N. Price, Robert Jordan Waters, Jayashree Ray, Hans K. Carlson, Jacob S. Lamson, ...
https://www.researchgate.net/publication/305479119_MiniTn7-transposon_delivery_vectors_for_inducible_or_constitutive_fluorescent_protein_expression_in_Enterobacteriaceae
Jul 20, 2016 · MiniTn7-transposon delivery vectors for inducible or constitutive fluorescent protein expression in Enterobacteriaceae Article (PDF Available) in …
https://www.sciencedirect.com/science/article/pii/S0167701209001857
Two new mariner transposon delivery vector systems, pMarA333 and pMarB333, had been constructed to randomly mutagenize Bacillus thuringiensis in vivo.The results showed that both systems could randomly insert into the genome of B. thuringiensis YBT881 (CCAM 020673). These systems could have potential for further construction of mutant libraries of B. thuringiensis and other Bacillus strains.Author: Mingshun Li, Minglei Li, Wen Yin, Jin He, Ziniu Yu
https://academic.oup.com/pcp/article/55/11/2017/2756049
Sep 16, 2014 · Therefore, an in vivo transposon mutagenesis system is another effective tool even for the naturally transformable cyanobacteria because only Escherichia coli harboring proper plasmids is needed for the conjugal transfer of a transposon delivery vector. The in vivo transposon mutagenesis of cyanobacteria has been developed mainly for Anabaena ...Author: Kazuyuki Watabe, Mamoru Mimuro, Tohru Tsuchiya
https://www.biorxiv.org/content/10.1101/158840v1
Jul 03, 2017 · Transposon mutagenesis coupled to next-generation sequencing (TnSeq) is a powerful approach for discovering the functions of bacterial genes. However, the development of a suitable TnSeq strategy for a given bacterium can be costly and time-consuming. To meet this challenge, we describe a parts-based strategy for constructing libraries of hundreds of transposon delivery vectors, which we …Author: Hualan Liu, Morgan N. Price, Robert Jordan Waters, Jayashree Ray, Hans K. Carlson, Jacob S. Lamson, ...
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