Transposon Delivery Plasmid

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New transposon delivery plasmids for insertional ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175031/
    Two new transposon delivery vector systems utilizing Mariner and mini-Tn10 transposons have been developed for in vivo insertional mutagenesis in Bacillus anthracis and other compatible Gram-positive species. The utility of both systems was directly demonstrated through the mutagenesis of a widely used B. anthracis strain.Author: Adam C. Wilson, Marta Perego, James A. Hoch

New transposon delivery plasmids for insertional ...

    https://www.sciencedirect.com/science/article/pii/S0167701207003144
    Transposon delivery plasmids. A. pAW016, the mini-Tn10 delivery plasmid. CmR, chloramphenicol-resistance cassette; SpcR, spectinomycin-resistance cassette; IR, invert repeat transposase recognition sites. Vector constructed from source plasmids as indicated in text. B. pAW068, the Mariner transposon delivery plasmid. C.Author: Adam C. Wilson, Marta Perego, James A. Hoch

Transposon Mutagenesis - an overview ScienceDirect Topics

    https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/transposon-mutagenesis
    Once a temperature‐sensitive strain has been obtained the strain can be the subject of a transposon mutagenesis. We utilized the mini‐ Tn10 delivery plasmid pIC333 (Steinmetz and Richter, 1994). This plasmid features an ery R marker, a thermosensitive gram‐positive origin of replication, and the transposase gene.

Hybrid Nonviral/Viral Vector Systems for Improved piggyBac ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395777/
    Feb 03, 2015 · Recombinant piggyBac transposon and transposase are typically codelivered by plasmid transfection; however, plasmid delivery is inefficient in somatic cells in vivo and is a barrier to the therapeutic application of transposon-based vector systems.Author: Ashley L Cooney, Brajesh K Singh, Patrick L Sinn

Addgene: Transposon cloning vectors

    https://www.addgene.org/browse/article/3690/
    Addgene is operating at a reduced capacity. Pending order? We will email to confirm that your organization can accept shipments. Conducting coronavirus or COVID-19 research?

High-efficiency transposon mutagenesis by electroporation ...

    https://academic.oup.com/femsle/article/153/2/363/585512
    Aug 01, 1997 · Abstract. A method is described for mutagenesis of Pseudomonas fluorescens strains by electroporation with the transposon delivery vector pUT/mini-Tn5 Km. The transposition process was shown to be optimal at 12.5 kV cm −1 for a pulse time (Bowen and Koslak, 1992) of about 4 ms. The Pseudomonas fluorescens L6.5 target strain exhibited maximal electrocompetence when harvested at …Author: François Artiguenave, Roland Vilaginès, Claude Danglot

pSC189 - Addgene

    https://www.addgene.org/32114/
    Plasmid pSC189 from Dr. Eric Rubin's lab is published in Gene. 2002 Aug 21;296(1-2):179-85. This plasmid is available through Addgene.

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