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https://www.ncbi.nlm.nih.gov/pubmed/18537656
The design of vectors for RNAi delivery system. Wang QZ(1), Lv YH, Diao Y, Xu R. Author information: (1)Institute of Molecular Medicine, Huaqiao University, China 362021. RNA interference (RNAi) is a process of double-stranded RNA-dependent post-transciptional gene silencing that occurs mainly in the mRNA processing bodies (P-bodies) of cells.Author: Qi Zhao Wang, Ying Hui Lv, Yong Diao, Ruian Xu
http://www.eurekaselect.com/openurl/content.php?genre=article&issn=1381-6128&volume=14&issue=13&spage=1327
The RNAi delivery systems can be either non-viral or viral vectors. The main candidate for RNAi as a therapeutical tool is the viral-based vectors, including retroviruses, adenoviruses, adeno-associated viruses (AAV), lentiviruses and herpes simplex virus-1 (HSV-1).
https://www.thermofisher.com/us/en/home/life-science/rnai/vector-based-rnai.html
Vector-Based RNAi and shRNA - RNAi in any cell type, even hard to transfect, ... Only delivery can be tracked (with separate expression cassette) Compatible with most Gateway® DEST vectors: Compatible with BLOCK-iT™ DEST Vectors: Best design success (>70%) Moderate design success (~50%) Table 2. Our RNAi vector products.
https://www.sciencedirect.com/science/article/pii/S0169409X19300547
Extensive SAR studies have provided design criteria, as described above. Moreover, formulation methods are also very important to the efficacy in vivo. Direct conjugation of small molecule ligands or polymers to siRNA offers the advantage of a being single component delivery system …Author: Yizhou Dong, Daniel J. Siegwart, Daniel G. Anderson
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2977003/
Jun 11, 2010 · RNAi represents a promising new approach for producing gene-specific inhibition and knockouts, producing transgenic animal models, and designing new therapeutics. This field is progressing at a very rapid pace. The mechanisms of RNAi were first reported in Caenorhabditis elegans in 1998 . The high significance and transformative values of this ...Author: Jie Wang, Ze Lu, M. Guillaume Wientjes, Jessie L.-S. Au
https://www.researchgate.net/publication/331215611_Design_of_AAV_Vectors_for_Delivery_of_RNAi_Design_and_Delivery
Design of AAV Vectors for Delivery of RNAi: Design and Delivery Chapter in Methods in molecular biology (Clifton, N.J.) 1950:3-18 · January 2019 with 48 Reads How we measure 'reads'
https://www.creative-biolabs.com/gene-therapy/adenoviral-vector-design-for-rnai-delivery.htm
Most adenoviral vectors used in RNAi delivery are derived from human serotypes 2 and 5, the commonly used adenoviruses in gene therapy. In general, siRNA delivery strategy is driven by H1-RNA promoter, while RNA pol II CMV promoter has been demonstrated to mediate gene silencing both in vitro and in vivo. The adenoviral vector expressing si/sh ...
https://www.sciencedirect.com/science/article/pii/S2214574518301482
RNAi is envisaged not as a standalone pest control tool but rather as part of a synergistic approach. Critical to the success and safety of insect-targetting RNAi is the appropriate choice of delivery method. At present the most exciting innovations are the use of nanoparticles, and the expression of trigger RNAs by a range of microbes.Author: Miranda Ma Whitten
https://www.thermofisher.com/in/en/home/life-science/rnai/vector-based-rnai/shrna-vectors.html
Transfer your shRNA cassette into one or more of the BLOCK-iT DEST vectors to get efficient delivery and expression of shRNA in mammalian cells. Each destination (DEST) vector is designed with GatewayTechnology to easily transfer the RNAi cassette from the BLOCK-iT pENTR vector to a BLOCK-iT-DEST vector in just one hour.
http://veritasbiollc.com/rna-delivery-technology/
Delivery of RNAi: A novel approach for delivery of Nucleic Acids to Cells. Provides a solution to the rate-limiting step in the delivery of RNA-based drugs (RNAi, miRNA, mRNA).. Background: Although, RNAi and antisense technologies have the potential to be developed as targeted therapeutics, delivery of these nucleic acid molecules has been a challenge.
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