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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679364/
While siRNA delivers the siRNA duplex directly to the cytosol, shRNAs are capable of DNA integration and consist of two complementary 19–22 bp RNA sequences linked by a short loop of 4–11 nt similar to the hairpin found in naturally occurring miRNA.Author: Chris B. Moore, Elizabeth H. Guthrie, Max Tze-Han Huang, Debra J. Taxman
https://www.sciencedirect.com/topics/neuroscience/small-hairpin-rna
Short Hairpin RNA Short hairpin RNA (shRNA) is an alternative way to prepare siRNA sequences for delivery to cells that can be expressed in situ from plasmid DNA (pDNA) or from virus-derived constructs.
https://geneticeducation.co.in/what-is-shrna-short-hairpin-rna/
The shRNA is called a short hairpin RNA or small hairpin RNA, is 20 to 25 bp polynucleotide chain of the RNA in which 4 to 11 nucleotides forms a loop, a hairpin-like loop. Although it does not have the dinucleotide overhang at the 3’ OH end of it, unlike the siRNA.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949849/
Sep 21, 2010 · Perfectly complementary dsRNA (short hairpin RNA, shRNA) is chopped up by Dicer, a ribonuclease III (RNase III) family member, into small interfering RNA (siRNA) duplexes 21-23 nt in length with symmetric 2-3 nucleotide (nt) 3' overhangs [ 3 ]. The use of siRNA duplexes is often accompanied by off-target effects,...Author: Katja Sliva, Barbara S Schnierle
https://www.researchgate.net/publication/46392655_Selective_gene_silencing_by_viral_delivery_of_short_hairpin_RNA
Selective gene silencing by RNAi can be achieved essentially by two nucleic acid based methods: i) cytoplasmic delivery of short double-stranded (ds) interfering RNA oligonucleotides …
http://hairpintechnologies.com/shrna-technology.html
A small hairpin RNA or short hairpin RNA(shRNA) is a sequence of RNA that makes a tight hairpin turn that can be used to silence target gene expression via RNA interference (RNAi). shRNA are synthesized in the nucleus of cells, further processed and transported to the cytoplasm and then incorporated into the RNA-interfering silencing complex (RISC) for activity.
http://www.jbc.org/content/282/46/33562.full
Nov 16, 2007 · Short hairpin RNA-mediated down-regulation led to similar (about 70%) decrease of both NADK expression, activity, and the NADPH concentration and was accompanied by increased sensitivity toward H 2 O 2.
https://www.labome.com/method/siRNAs-and-shRNAs-Tools-for-Protein-Knockdown-by-Gene-Silencing.html
Short hairpin RNA (shRNA) that contains a loop structure that is processed to siRNA and also leads to the degradation of mRNAs in a sequence-specific manner dependent upon complementary binding of …Author: Erin P O’Keefe
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