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https://www.thermofisher.com/us/en/home/life-science/rnai/delivering-rnai-to-cells-transfection-and-viral-delivery.html.html
Selecting transfection reagents and viral delivery methods Specially designed and manufactured for deliver of dsRNA (siRNA or Stealth RNAi siRNA). Superior efficiencies allow low concentrations of siRNA to be used. Mild cytotoxicity profile facilitates optimization. Wide range of compatibility ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112363/
Nov 17, 2016 · Delivery of dsRNA using chemically modified molecules, polymer nanoparticles, liposomes, viruses or bacteria, could increase efficacy in attaining a potent RNAi response. The choice of the delivery method and the choice of formulation would of course depend on the circumstances, on the target insect and on the reason for impaired RNAi-efficiency.Author: Mallikarjuna R. Joga, Moises J. Zotti, Guy Smagghe, Olivier Christiaens
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2977003/
Jun 11, 2010 · RNA interference (RNAi) is a highly efficient regulatory process that causes posttranscriptional gene silencing in most eukaryotic cells . RNAi represents a promising new approach for producing gene-specific inhibition and knockouts, producing transgenic animal models, and designing new therapeutics. This field is progressing at a very rapid pace.Author: Jie Wang, Ze Lu, M. Guillaume Wientjes, Jessie L.-S. Au
https://www.pbs.org/wgbh/nova/article/rnai-cure/
Jul 01, 2005 · But the delivery method McCaffrey and Kay used, which doubled the mice's blood volume within eight seconds, isn't feasible for humans. And even if it were, the effects of naked RNA …
https://www.jove.com/video/57390/double-stranded-rna-oral-delivery-methods-to-induce-rna-interference
This vegetable mediated dsRNA delivery protocol has been used successfully to induce RNAi not only in BMSB but also in HB using collard greens (Figure 3), though delivery strategies and methods must be optimized for each gene and insect. Hence, it is recommended that various methods be tested for delivery as well as to target multiple genes of interest individually or by stacking at least two dsRNAs …Author: Saikat Kumar B. Ghosh, Wayne B. Hunter, Alexis L. Park, Dawn E. Gundersen-Rindal
https://www.nature.com/articles/d41586-019-03071-9
Oct 16, 2019 · “The conundrum of RNA therapeutics has always been delivery,” says Bo Rode Hansen, chief executive of Genevant Sciences, a company in Cambridge, Massachusetts, that focuses on delivery methods ...Author: Bianca Nogrady
http://altogenlabs.com/rna-interference-rnai-services/in-vivo-sirna-delivery-and-tissue-targeting/
Altogen Labs has developed methods to prevent siRNA degradation in serum (including both chemical modification and encapsulation of siRNA), increase delivery efficiency to the target organ, and produce strong tissue-targeted functional effects induced by siRNA in vivo.
https://genomemedicine.biomedcentral.com/articles/10.1186/s13073-017-0450-0
Jun 27, 2017 · Nanoparticle encapsulation of RNA physically protects nucleic acids from degradation and, depending on the specific chemistry, can aid in cellular uptake and endosomal escape. Given their high degree of chemical flexibility, polymers are commonly used …Author: James C. Kaczmarek, Piotr S. Kowalski, Daniel G. Anderson
https://www.thermofisher.com/us/en/home/life-science/rnai/rna-interference-overview.html
RNAi has had significant impact on the ease, speed, and specificity with which the loss of gene function analysis can be done in mammalian cells and animal models. This is becoming the method of choice for researchers doing loss of function studies. Gene over expression has been used to analyze the function...
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