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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2857543/
The PLGA microspheres for protein delivery can be prepared by several methods with the most widely used techniques for proteins conjugation being: (i) spray drying, (ii) double emulsion, and (iii) phase separation-coacervation. 92Author: Dipak S. Pisal, Matthew P. Kosloski, Sathy V. Balu-Iyer
https://www.sciencedirect.com/science/article/pii/S0958166900002020
In the future, the success of drug development will depend upon innovative methods for protein delivery. The key drivers for selecting a protein delivery method for commercial development include market competition, patient convenience and compliance, requirement for local or topical delivery and systemic toxicity or other safety issues.Author: Jeffrey L Cleland, Ann Daugherty, Randall Mrsny
https://www.nature.com/articles/nmeth.2998
Jun 15, 2014 · Delivery did not require a binding interaction between dfTAT and a protein, multiple molecules could be delivered simultaneously, and delivery could be repeated. dfTAT-mediated delivery …Author: Alfredo Erazo-Oliveras, Kristina Najjar, Laila Dayani, Ting-Yi Wang, Gregory A Johnson, Jean-Philipp...
https://www.sciencedirect.com/science/article/pii/S1773224715300058
Academic and industrial researchers have presented and investigated new polymers for protein delivery such as hydroxy-ethyl-starch , polyoxazoline , , , dextrin , polysialic acid , , hyaluronic acid , , , , polypeptides (XTEN technology , PASylation ), etc. While biodegradable polymers are desirable for drug delivery, biodegradability is not easily controlled.Author: Antonella Grigoletto, Katia Maso, Anna Mero, Antonio Rosato, Oddone Schiavon, Gianfranco Pasut
https://www.takarabio.com/learning-centers/gene-function/gene-editing/crispr/cas9-delivery-methods
CRISPR/Cas9 delivery methods. For gene editing in mammalian cells we typically recommend delivery of a ribonuclear protein (RNP) complex consisting of Cas9 protein and a single guide RNA (sgRNA). RNPs remain in the cell for a short time and the dose is minimal, leading to lower toxicity and reduced editing at off-target sites compared...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3956587/
For more information on the use of B12 to improve the oral delivery of protein and peptides, please see the review by Petrus et al. . Protein lipidization is another method that increases the bioavailability of orally administered proteins.Author: Benjamin J Bruno, Geoffrey D Miller, Carol S Lim
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