We have collected information about Protein Delivery Into Cells for you. Follow the links to find out details on Protein Delivery Into Cells.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151968/
Oct 16, 2007 · In conclusion, we have presented a practical approach for protein delivery into live cells and we have demonstrated that the determination of optimal conditions is required for efficient delivery. Routinely, we recommend using 1–2 μg of R-PE and 2–4 μL of …Author: Claire O. Weill, Stéphanie Biri, Abdennaji Adib, Patrick Erbacher
https://pubs.acs.org/doi/10.1021/jacs.8b10299
The efficient delivery of proteins into cells is needed to fully realize the potential of protein-based therapeutics. Current protein delivery strategies generally suffer from poor endosomal escape and low tolerance for serum. Here, the genetic fusion of a supercharged polypeptide, called SCP, to a protein provides a generic method for intracellular protein delivery. It allows efficient ...Author: Jun Yin, Qun Wang, Shan Hou, Lichen Bao, Wenbing Yao, Xiangdong Gao
https://www.ncbi.nlm.nih.gov/pubmed/30398334
Dec 12, 2018 · Here, the genetic fusion of a supercharged polypeptide, called SCP, to a protein provides a generic method for intracellular protein delivery. It allows efficient protein endocytosis and endosomal escape and is capable of potently delivering various proteins with a range of charges, sizes, and bioactivities into the nucleus of living cells.Author: Jun Yin, Qun Wang, Shan Hou, Lichen Bao, Wenbing Yao, Xiangdong Gao
https://www.cell.com/cell/fulltext/S0092-8674(15)00315-3
Apr 23, 2015 · Here, we describe a method for the efficient delivery of native proteins and other macromolecules, such as small RNAs, into primary cells. We discovered that a combination of small molecules drives the highly efficient intracellular delivery of native proteins, independent of any transduction peptide.Author: Diego S. D’Astolfo, Romina J. Pagliero, Anita Pras, Wouter R. Karthaus, Hans Clevers, Vikram Prasad,...
https://www.cell.com/trends/cell-biology/comments/S0962-8924(00)01771-2
For instance, a fusion between TAT and herpes-simplex-virus TK protein will transduce into cells and has its highest enzymatic activity when purified from the soluble bacterial fraction and not from the 8-m-urea-denatured fraction. Similarly, TAT–β-galactosidase becomes refolded and assembled into an enzymatically active homotetramer when ...Author: Steven R. Schwarze, Keith A. Hruska, Steven F. Dowdy
https://science.sciencemag.org/content/284/5418/1322.full
May 21, 1999 · Several Gram-negative pathogenic bacteria have evolved a complex protein secretion system termed type III to deliver bacterial effector proteins into host cells that then modulate host cellular functions. These bacterial devices are present in both plant and animal pathogenic bacteria and are evolutionarily related to the flagellar apparatus.Author: Jorge E. Galán, Alan Collmer
https://www.frontiersin.org/articles/10.3389/fpls.2017.00519/full
Apr 19, 2017 · Cell bombardment has also been used successfully for DNA delivery into plant cells and tissues. However, success with this technique is limited by the amount of material that can be loaded on a particle and the ratio of surviving cells. For our study, we have opted for electroporation to deliver the protein of interest into plant cells.Author: Cesyen Cedeño, Kris Pauwels, Peter Tompa, Peter Tompa
Searching for Protein Delivery Into Cells?
You can just click the links above. The data is collected for you.
