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https://www.researchgate.net/publication/44670615_Potent_Delivery_of_Functional_Proteins_into_Mammalian_Cells_in_Vitro_and_in_Vivo_Using_a_Supercharged_Protein
Potent Delivery of Functional Proteins into Mammalian Cells in Vitro and in Vivo Using a Supercharged Protein Article in ACS Chemical Biology 5(8):747-52 · August 2010 with 34 Reads
https://www.nature.com/articles/nbt.3081
Oct 30, 2014 · Cronican, J.J. et al. Potent delivery of functional proteins into mammalian cells in vitro and in vivo using a supercharged protein. ACS Chem. Biol. 5 , 747–752 (2010). CAS
https://www.cell.com/cell-chemical-biology/fulltext/S2451-9456(17)30228-3
Delivery of exogenous proteins to mammalian cells can sometimes occur via direct membrane translocation, resulting in high levels of cytosolic residence of internalized cargo. Often, however, delivery occurs through one of the many well-studied pathways for the uptake of exogenous material (including endocytosis, micropinocytosis, and ...
http://core.ac.uk/display/8443819
Potent Delivery of Functional Proteins into Mammalian Cells in Vitro and in Vivo Using a Supercharged Protein By James J. Cronican, David B. Thompson, Kevin T. Beier, Brian R. McNaughton, Constance L. Cepko and David R. Liu
https://www.sciencedirect.com/science/article/pii/S2451945617302283
Inside Job: Methods for Delivering Proteins to the Interior of Mammalian Cells ... researchers have dedicated an enormous amount of effort to developing methods for exogenous protein delivery to mammalian cells. Some particularly popular methods are reviewed below. ... A class of human proteins that deliver functional proteins into mammalian ...
https://dash.harvard.edu/bitstream/handle/1/29003557/nihms-316503.pdf?sequence=1
Functional Protein Delivery into Mammalian Cells in vivo We next sought to determine if this class of proteins could serve as a natural platform for the delivery of macromolecular cargo in vivo. The direct delivery of functional protein into cells in vivo is of special interest because of the rarity of successful in vivo protein transduction
https://core.ac.uk/display/154868578
Potent Delivery of Functional Proteins into Mammalian Cells in Vitro and in Vivo Using a Supercharged Protein By James J. Cronican, David B. Thompson, Kevin T. Beier, Brian R. McNaughton, Connie Cepko and David Ruchien Liu
https://www.researchgate.net/publication/334177587_An_anionic_human_protein_mediates_cationic_liposome_delivery_of_genome_editing_proteins_into_mammalian_cells
Delivery into mammalian cells remains a significant challenge for many applications of proteins as research tools and therapeutics. ... J. J. et al. Potent delivery of functional proteins into ...
https://www.acs.org/content/acs/en/pressroom/presspacs/2010/acs-presspac-july-14-2010/supercharged-proteins-enter-forbidden-zone-of-biology.html
The new technique, described in the monthly journal, ACS Chemical Biology: “Potent Delivery of Functional Proteins into Mammalian Cells in Vitro and in Vivo Using a Supercharged Protein,” represents a new use for an engineered form of green fluorescent protein, the topic of the 2008 Nobel Prize in Chemistry that is one of the most important ...
https://www.jove.com/video/52814/direct-protein-delivery-to-mammalian-cells-using-cell-permeable-cys2
Cronican, J. J., et al. Potent delivery of functional proteins into mammalian cells in vitro and in vivo using a supercharged protein. ACS Chem. Biol. 5, 747-752 (2010). Panyam, J., Labhasetwar, V. Biodegradable nanoparticles for drug and gene delivery to cells and tissue. Adv. Drug Deliv. Rev. 55, …
https://currentprotocols.onlinelibrary.wiley.com/doi/full/10.1002/cpps.77
Sep 28, 2018 · The viruses are generated and amplified in insect cells and are then used to infect mammalian cells, where the target protein will be produced. Improved BacMam vectors have been generated, expressing vesicular stomatitis virus glycoprotein G in the viral envelope to make the infection of mammalian cells more efficient.
https://mbio.asm.org/content/9/6/e02321-18
Anti-CRISPR proteins interact with NmeCas9 in mammalian cells to inhibit genome editing. (A) Anti-CRISPR proteins interact with NmeCas9 in HEK293T cells. Pulldowns of FLAG-tagged Acr and coimmunoprecipitated, HA-tagged NmeCas9 are confirmed by Western blotting. As a negative control, an untagged version of Acrs was used for pulldown.
https://link.springer.com/protocol/10.1007%2F978-1-4939-8799-3_9
Aug 29, 2018 · Cronican JJ, Thompson DB, Beier KT, McNaughton BR, Cepko CL, Liu DR (2010) Potent delivery of functional proteins into mammalian cells in vitro and in vivo using a supercharged protein. ACS Chem Biol 5:747–752 CrossRef PubMed PubMedCentral Google Scholar
https://www.thno.org/v08p3474.pdf
lethal-7a, was delivered to cells with high efficiency and safety, bypassing the endo -lysosomal system [25] . In this study, based on these previous findings, we hypothesized that PNPs can achieve potent intracellular delivery of functional proteins a via non-endo-lysosomal pathway for cancer treatment (Scheme 1). This drug-delivering-drug ...
https://en.wikipedia.org/wiki/David_R._Liu
David Ruchien Liu (born 1973) is an American chemist and biologist. He is the Richard Merkin Professor, Director of the Merkin Institute of Transformative Technologies in Healthcare, and Vice-Chair of the Faculty at the Broad Institute of Harvard and MIT; …
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