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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4347098/
Jan 01, 2015 · The optimal vector and delivery system depends on the target cells and its characteristics, duration of expression and the size of the genetic material to be incorporated in the vector [3,4].The present vectors used for gene therapy are broadly classified as Viral vectors, Non-viral vectors and engineered vectors. The non-viral vectors are Naked DNA, particle based and …Author: Murali Ramamoorth, Aparna Narvekar
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782077/
Oct 16, 2009 · These improvement will rely on our better understanding of the limiting steps that nonviral vector must overcome. Developing new vectors that are more target specific will also be necessary. The strategies that merge nonviral and viral vectors might be helpful to achieve more, efficient, long-lasting, and nontoxic gene delivery systems.Author: Mohammed S. Al-Dosari, Xiang Gao
https://www.intechopen.com/books/gene-therapy-tools-and-potential-applications/non-viral-delivery-systems-in-gene-therapy
Cationic SLN, for instance, SLN containing at least one cationic lipid, have been proposed as non-viral vectors for gene delivery [105,20]. It has been shown that cationic SLN can effectively bind nucleic acids, protect them from DNase I degradation and deliver them into living cells.Author: Alicia Rodríguez Gascón, Ana del Pozo-Rodríguez, María Ángeles Solinís
https://www.nature.com/articles/nrg3763
Jul 15, 2014 · Non-viral vectors for gene-based therapy. Hao Yin Hao Yin is a postdoctoral associate at Massachusetts Institute of Technology, Cambridge, USA. He received his B.S. in biochemistry at Nanjing University, China, and his Ph.D. in toxicology from the School of Pharmacy, University of Colorado Anschutz Medical Campus, Aurora, USA.Author: Hao Yin, Rosemary L. Kanasty, Ahmed A. Eltoukhy, Arturo J. Vegas, Arturo J. Vegas, J. Robert Dorkin,...
https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(18)30019-4
Apr 04, 2018 · non-viral gene delivery into HSPCs is still considered to be inefficient when compared with viral technologies. In addition, such a physical way of naked plasmid DNA delivery into HSPCs results in an excessive loss of cell viability, and the observed cytotoxicity increases proportionally to plasmid DNA load.Author: Marta Holstein, Cristina Mesa-Nuñez, Csaba Miskey, Elena Almarza, Valentina Poletti, Marco Schmeer, ...
https://www.the-scientist.com/news/viral-vs-nonviral-in-gene-therapy-which-vector-will-prevail-56938
Jun 22, 1998 · The director of the University of Alabama at Birmingham's gene therapy program also noted that viral vectors have generated more research activity than nonviral vectors. However, nonviral backers think their delivery vehicles for therapeutic genetic material promise greater impact in …
http://www.genetherapynet.com/non-viral-vectors.html
Non-Viral Vectors Non-viral methods present certain advantages over viral methods, with simple large scale production and low host immunogenicity being just two. Previously, low levels of transfection and expression of the gene held non-viral methods at a disadvantage; however, recent advances in vector technology have yielded molecules and techniques with transfection efficiencies similar to …
https://www.slideshare.net/Aparajitha_Anne/gene-delivery-system
May 19, 2012 · GENE DELIVERY:• In most gene delivery studies a normal gene is inserted into the genome to replace an abnormal disease causing gene.• A carrier molecule called vector carries the therapeutic gene to patient’s target cells. Vectors generally used are viruses.•
https://www.slideshare.net/syeimy/gene-therapy-ppt
Feb 20, 2012 · BASIC PROCESS OF GENE THERAPY 1. VIRAL VECTOR 2. NON VIRAL VECTOR 9. GT utilizes the delivery of DNA into cells, which can be accomplished by a number of methods. The two major classes of methods : recombinant viruses – VIRAL VECTOR naked DNA or DNA complexes – NONVIRAL VECTOR 10.
https://www.youtube.com/watch?v=B0zggFZvH9M
Dec 09, 2013 · This gene therapy video tutorial focuses on different non viral gene delivery system to deliver normal copy of gene inside host tissue to cure genetic diseases. For more information, log on to ...Author: Shomu's Biology
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