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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · Viral and nonviral delivery systems for gene delivery. ... Chemical delivery, gene therapy, non viral delivery systems, ... There are different viral and nonviral vectors for gene delivery, but all gene therapy applications depend on the fact that the genetic material needs to be delivered across the cell membrane and ultimately to the cell ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4347098/
Jan 01, 2015 · The optimal vector and delivery system depends on the target cells and its characteristics, duration of expression and the size of the genetic material to be incorporated in the vector [3,4].The present vectors used for gene therapy are broadly classified as Viral vectors, Non-viral vectors and engineered vectors. The non-viral vectors are ...Author: Murali Ramamoorth, Aparna Narvekar
https://pubs.acs.org/doi/10.1021/cr800409e
Dec 03, 2008 · Cationic Amino Acid Based Lipids as Effective Nonviral Gene Delivery Vectors for Primary Cultured Neurons. ACS Chemical Neuroscience 2013, 4 (12) , 1514-1519. DOI: 10.1021/cn400036j. Yang Yang, Ying-Ming Zhang, Yong Chen, Jia-Tong Chen, and Yu Liu .Author: Meredith A. Mintzer, Eric E. Simanek
https://www.intechopen.com/books/gene-therapy-tools-and-potential-applications/non-viral-delivery-systems-in-gene-therapy
Gene delivery systems include viral vectors and non-viral vectors. Viral vectors are the most effective, but their application is limited by their immunogenicity, oncogenicity and the small size of the DNA they can transport. Non-viral vectors are safer, of low cost, more reproducible and do not present DNA size limit.Author: Alicia Rodríguez Gascón, Ana del Pozo-Rodríguez, María Ángeles Solinís
https://www.intechopen.com/books/recent-advances-in-novel-drug-carrier-systems/gene-delivery-systems-recent-progress-in-viral-and-non-viral-therapy
Viral-mediated gene delivery systems consist of viruses that are modified to be replication-deficient, but which can deliver DNA for expression. Adenoviruses, retroviruses, and lentiviruses are used as viral gene-delivery vectors (Escors and Breckpot, 2010). Non-viral gene delivery systems were introduced as an alternative to viral-based systems.Author: Erdal Cevher, Ali Demir Sezer, Emre Şefik Çağlar
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/transfection-basics/gene-delivery-technologies.html
Can be used for in vivo delivery of nucleic acids; Can be used for making stable cell lines (retroviral vectors) or for transient expression (adenoviral vectors) Cell lines to transfect must contain viral receptors; Limited insert size (~10 kb for most viral vectors versus ~100 kb for non-viral vectors)
http://www.genetherapynet.com/non-viral-vectors.html
Non-viral methods present certain advantages over viral methods, with simple large scale production and low host immunogenicity being just two. Previously, low levels of transfection and expression of the gene held non-viral methods at a disadvantage; however, recent advances in vector technolog...
https://www.nature.com/articles/nrg3763
Jul 15, 2014 · Synthetic delivery vectors have the potential to address many of the limitations of viral vectors, particularly with respect to safety. For systemic delivery of DNA, both lipid-based vectors and ...Author: Hao Yin, Rosemary L. Kanasty, Ahmed A. Eltoukhy, Arturo J. Vegas, Arturo J. Vegas, J. Robert Dorkin,...
https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/non-viral-vector
Non-viral vectors. Gene delivery based on non-viral vectors has attracted much attention due to their universal application range and high safety level of delivery. 5 A number of nanoparticle delivery systems based on polymers and liposomes have been developed. Furthermore, physical approaches including, for example, electroporation and gene ...
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