Mouse Intranasal Delivery Protocol

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Intranasal Administration of CNS Therapeutics to Awake Mice

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3653240/
    Apr 08, 2013 · Intranasal delivery to the CNS is an especially useful technique because not only is it rapid, but it targets the CNS reducing systemic exposure. For example, intranasal administration of deferoxamine to rats resulted in 3-19 times higher concentrations in brain regions with 28 times lower concentrations in the blood 11.Author: Leah R. Hanson, Jared M. Fine, Aleta L. Svitak, Katherine A. Faltesek

A Positioning Device for the Placement of Mice During ...

    https://www.jove.com/video/59201/a-positioning-device-for-placement-mice-during-intranasal-sirna
    Summary. Here, we present a protocol using a mouse positioning device that enables the appropriate placement of mice for the intranasal administration of a brain-targeting peptide-siRNA formulation enabling effective gene silencing in …Author: Irfan Ullah, Irfan Ullah, Kunho Chung, Jagadish Beloor, Sang-Kyung Lee, Priti Kumar

Intranasal Delivery of Therapeutic Stem Cells to ...

    https://www.jove.com/video/55845/intranasal-delivery-therapeutic-stem-cells-to-glioblastoma-mouse
    Stem cells are promising therapeutic carriers to treat brain tumors due to their intrinsic tumor tropism. Non-invasive intranasal stem cell delivery bypasses the blood brain barrier and demonstrates strong potential for clinical translation. This article summarizes the basic principles of intranasal stem cell delivery in a mouse …Author: Dou Yu, Gina Li, Maciej S. Lesniak, Irina V. Balyasnikova

Intranasal infection of mice - YouTube

    https://www.youtube.com/watch?v=u2F6UU0dQw0
    Jan 27, 2015 · A Wicked Harpoon Eyed Spring Loaded Mouse Trap From The Civil War Era. Mousetrap Monday - Duration: 11:08. Shawn Woods Recommended for you. NewAuthor: Dilini Ranatunga

Dear colleagues, please tell us how to do intranasal ...

    https://www.researchgate.net/post/Dear_colleagues_please_tell_us_how_to_do_intranasal_administration_to_mice_methodHow_to_minimize_animal_stress
    Dear colleagues, please tell us how to do intranasal administration to mice (method)?How to minimize animal stress? ... Does anyone have a good protocol for how to collect and process the samples ...

Intranasal immunization of mice - YouTube

    https://www.youtube.com/watch?v=2aDykvOxBWk
    Nov 22, 2016 · The anesthetized mouse was immunized intranasally with up to 20 μl (10 μl per nostril) antigen. ... Intranasal immunization of mice Sobhan Faezi. Loading... Unsubscribe from Sobhan Faezi?Author: Sobhan Faezi

Protocol for intranasal inoculation of mice

    https://www.uab.edu/sites/luckielab/protocols/ininnoc.doc
    After the mouse is deeply anaesthetized (confirmed by the absence of reflexes on the footpad), the mouse is held by it’s ears and 20 ul of the inoculum is gradually released into the nostrils (10 ul in each nostril) with the help of a micropipette. ... Protocol for intranasal inoculation of mice ...

Intranasal drug delivery procedure

    http://intranasal.net/documentstoload/Intranasalmedicationdeliveryprotocolphotoguide1.pdf
    Intranasal drug delivery procedure Basic Intranasal Medication Delivery Materials: 1. Syringe and needle/needleless device to draw up the medication 2. Atomizer 3. Medication of appropriate concentration for nasal medication delivery High concentration - Low volume Ideal volume 0.2 to 0.3 ml per nostril Maximum volume 1 ml per nostril Procedure: 1.

Adenovirus Cre Intranasal Infection Jacks Lab

    https://jacks-lab.mit.edu/protocols/adenovirus_cre_intranasal_infection
    If you notice a mouse that is breathing really slowly or shivering, filling a large glove with hot water and placing the mouse on the glove often helps it to recover & survive. Adeno-Cre or Lenti-Cre Protocol. See: Dupage M*, Dooley AL*, Jacks T. 2009. Conditional mouse lung tumor models using adenoviral or lentiviral delivery of Cre recombinase.

Intranasal Delivery of miR-146a Mimics Delayed Seizure ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5294386/
    Intranasal Delivery of miR-146a Mimics Delayed Seizure Onset in the Lithium-Pilocarpine Mouse Model. ... In the current study, a C57BL/6 TLE mouse model was established using the lithium-pilocarpine protocol. The seizure degree was evaluated according to the Racine scale, and level 5 was considered the threshold for generalized convulsions ...Author: Hua Tao, Jianghao Zhao, Tingting Liu, Yujie Cai, Xu Zhou, Huaijie Xing, Yan Wang, Mingkang Yin, Wang...

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