Mini-Tn7 Delivery Plasmids

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(PDF) MiniTn7-transposon delivery vectors for inducible or ...

    https://www.researchgate.net/publication/305479119_MiniTn7-transposon_delivery_vectors_for_inducible_or_constitutive_fluorescent_protein_expression_in_Enterobacteriaceae
    Jul 20, 2016 · MiniTn7-transposon delivery vectors for inducible or constitutive fluorescent protein expression in Enterobacteriaceae ... delivery plasmids in combination with the conjugation strain ...

MiniTn7-transposon delivery vectors for inducible or ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4972447/
    The use of miniTn7-transposon delivery plasmids is widespread in molecular and environmental microbiology, e.g. to chromosomally integrate promoter-reporter gene constructs (Choi and Schweizer 2006, McKenzie and Craig 2006, Lagendijk et al. 2010) or for the complementation of knockout mutants (Crepin, Harel and Dozois 2012).Author: Mitja N. P. Remus-Emsermann, Pascal Gisler, David Drissner

Mini-Tn7 transposons – the delivery plasmids

    http://comstat.dk/reswiki/lib/exe/fetch.php?media=tn7plasmids.pdf
    Mini-Tn7 delivery plasmids, ver. 1 1 Mini-Tn7 transposons – the delivery plasmids See also the documents: “The Tn7 transposon ” and “How to use the mini-Tn7 transposons”. Note ! The delivery plasmids presented here are all pUC19 derivatives and can replicate in E. coli and other enterics, but for example not in Pseudomonas. Only characters inserted as part

Mini‐Tn7 transposons for site‐specific tagging of bacteria ...

    https://www.researchgate.net/publication/8521456_Mini-Tn7_transposons_for_site-specific_tagging_of_bacteria_with_fluorescent_proteins
    We have expanded the panel of mini-Tn7 delivery plasmids expressing different fluorescent proteins (stable and unstable) from the Escherichia coli lac derived promoter, P(A1/04/03), or from the ...

Facts about the mini-Tn7 transposon system as a tool for ...

    http://comstat.dk/reswiki/lib/exe/fetch.php?media=tn7howto.pdf
    In addition, the plasmids contain a mob region, which can be used for mobilising the plasmid from one bacterium to another by the RP4/ RK2 transfer genes, e.g. located on the plasmid pRK600. Constructed delivery plasmids of this series are shown in the document:”Mini-Tn7 delivery plasmids”, see also Lambertsen et al., 2003. Helper plasmidFile Size: 176KB

mini-Tn7 insertion in bacteria with multiple glmS attTn7 ...

    https://apps.dtic.mil/dtic/tr/fulltext/u2/a450025.pdf
    mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344 Kyoung-Hee Choi1, David DeShazer2 & Herbert P Schweizer1 1Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523-1682, USA; 2Bacteriology Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, …Author: Kyoung-Hee Choi, David DeShazer, Herbert P Schweizer

Mini-Tn7 transposons for site-specific tagging of bacteria ...

    https://www.ncbi.nlm.nih.gov/pubmed/15186351
    The mini-Tn7 transposon system is a convenient tool for site-specific tagging of bacteria in which the tagging DNA is inserted at a unique and neutral chromosomal site. We have expanded the panel of mini-Tn7 delivery plasmids expressing different fluorescent proteins (stable and unstable) from the Escherichia coli lac derived promoter, P(A1/04 ...Author: Lotte Lambertsen, Claus Sternberg, Søren Molin

A simple method for construction of pir - BMC Research Notes

    https://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-5-157
    Mar 20, 2012 · Development of a mini-Tn7 based ori R6Kγ chromosomal insertion system. We sought to employ the mini-Tn7 method described by McKenzie and Craig [] for chromosomal insertion of pir alleles in the absence of selection. For this purpose, the pir + and pir-116 genes were cloned into the mini-Tn7 delivery vector pGRG36 (Figure 1) and published procedures [] followed in an attempt to transpose …Author: Brian H Kvitko, Steven Bruckbauer, John Prucha, Ian McMillan, Erin J Breland, Stephanie Lehman, Kati...

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