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https://health.mo.gov/lab/smearculture.php
The AFB culture is the inoculation of a clinical specimen onto culture media Becton-Dickinson Mycobacteria Growth Indicator Tube (B-D MGIT broth) and Lowenstein-Jensen (L-J) media slant, incubation at 37°C for up to six (6) weeks and detection of growth or no growth during this incubation period.
https://en.wikipedia.org/wiki/L%C3%B6wenstein%E2%80%93Jensen_medium
When grown on LJ medium, M. tuberculosis appears as brown, granular colonies (sometimes called "buff, rough and tough"). The medium must be incubated for a significant length of time, usually four weeks, due to the slow doubling time of M. tuberculosis (15–20 hours) compared with other bacteria.
The L&J Cafe is a uniquely flavorful link between El Paso’s past and present. Originally known as “Tony’s Place”, the restaurant opened on the outskirts of El Paso in 1927, providing home cooking, home brew and slot machines through Prohibition.
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