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https://www.nature.com/articles/3302688
Jan 05, 2006 · This review will focus on the development, lentiviral based delivery, and the potential therapeutic use of siRNAs in treating viral infections. ... or stable expression of the particular siRNA by ...Author: K V Morris, J J Rossi
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774780/
Thus, in order to apply this potent technique for in vivo studies and more specifically to obtain transgenic animals, efficient siRNA delivery methods must be developed . In this review, we describe the design of lentiviral vectors expressing shRNA and their use in generation …Author: Oded Singer, Inder M. Verma
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338230/
Small hairpin RNA (shRNA) and lentiviral vector. RNA interference has been used as a research tool to control the expression of specific genes in numerous experimental organisms and has potential as a therapeutic strategy to reduce the expression of problem genes[1,2].RNAi can be performed through a synthetic small interfering RNA (siRNA) or a vector-based shRNA. shRNAs, which are stem-loop ...Author: Hong Song, Ping-Chang Yang
https://www.cshlpress.com/pdf/sample/genetran.pdf
3 Development of Lentiviral Vectors Expressing siRNA Gustavo Tiscornia, Oded Singer, and Inder M. Verma The Salk Institute for Biological Studies, Laboratory of Genetics, La Jolla, California 92037 ABSTRACT This chapter describes the use of lentiviral vectors to deliver small interfering RNA (siRNA)-mediated silencing cassettes. TheFile Size: 260KB
https://www.thermofisher.com/us/en/home/life-science/rnai/delivering-rnai-to-cells-transfection-and-viral-delivery.html
The two common approaches for RNAi delivery are lipid-mediated transfection and viral-mediated transduction.Determining which one of these approaches to use depends on the cell type being studied and whether transient or stable knockdown is desired (Tables 1 & 2).
https://www.origene.com/products/cdna-clones/lentiviral-particles
Lentiviral vector systems for efficient DNA delivery, 3rd generation lenti vectors. Lenti-ORF clones and lenti shRNA in lentiviral plasmid and lentiviral particles. Many lenti videos and recorded webinars are available, regarding the biosafety of lentivirus, MOI optimization, how to package viral particles.
https://en.wikipedia.org/wiki/Short_hairpin_RNA
Lentiviral delivery of shRNA and the mechanism of RNA interference in mammalian cells. A short hairpin RNA or small hairpin RNA (shRNA/Hairpin Vector) is an artificial RNA molecule with a tight hairpin turn that can be used to silence target gene expression via RNA interference (RNAi).
https://www.researchgate.net/publication/10756045_Use_of_Lentiviral_Vectors_for_Delivery_of_Small_Interfering_RNA
A lentiviral delivery of an shRNA expression system has been developed as a new therapeutic strategy for the treatment of amyo- trophic lateral sclerosis ( Raoul et al., 2005).
https://www.researchgate.net/publication/7373319_Lentiviral-mediated_delivery_of_siRNAs_for_antiviral_therapy
Lentiviral vector delivery and expression of shRNAs targeting HIV-1. Lentiviral vectors can stably transduce target cells (1) integrate and constitutively express anti-HIV-1 siRNAs or shRNAs ...
https://www.thermofisher.com/us/en/home/life-science/rnai/virus-based-rnai-analysis/lentiviral-shrna-and-mirna-analysis.html
13 rows · Viral delivery can be the best option in virtually any mammalian cell type, including hard-to …
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