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https://hoursfinder.com/i-hours/ief-volt-hours.html
Volt-Hours 75 150 300 750 7500 - Voltage (V) 300 600 1500 3000 300 Time (H) 0.5 1 1 12 <20 Volt-Hours 150 600 1500 36000 - SymphonyIEF Flatbed IEF system for IPG strips and gels, with focusing and rehydration trays
http://www.alphametrix.de/electrophoresis/index.php?pageid=38
Volt-hours - Recommended Running Conditions for IEF of 18cm IPG strips: Voltage Step 1: Voltage (V) 300 Time (h) 1 Volt-hours 300: Voltage Step 2: Voltage (V) 600 Time (h) 1 Volt-hours 600: Voltage Step 3: Voltage (V) 1500 Time (h) 1 Volt-hours 1500: Voltage Step 4: Voltage (V) 3000 Time (h) 12,5 Volt-hours 37500: Voltage Step 5 end of run: Voltage (V) 330 Time (h) 20
http://www.topac.com/IEF-datasheet.pdf
CSL-IEF – Typical Running Conditions 7cm IPG Strip. IEF Step 12345 6 Voltage (V) 150 300 600 1500 3000 300 Time (h) 0.5 0.5 0.5 0.5 2.5 <20 Volt-hours 75 150 300 750 7500 -. 18cm IPG strip. IEF Step 12345 Voltage (V) 300 600 1500 3000 300 Time (h) 0.5 1 1 12 <20 Volt-hours 150 600 1500 36000 -. 1 2 5 3.
https://www.bio-rad.com/webroot/web/pdf/lsr/literature/4006164B.pdf
Time 00:01–99:00 hours:minutes or 0–99,999 volt-hours Focusing trays Material Polycarbonate Strip size 7, 17 cm Capacity 12 strips per tray 7cm 17 cm Electrode distance 64.5 mm 162 mm Accommodates total strip length 82.3 mm 181 mm Peltier platform Tray capacity One 17 cm tray or two 7 cm trays Temperature 10–25 °C ± 0.5 °C @ max. ambient of 30 °C
https://hercuvan.com/product/symphonyief-isoelectric-focusing/
IEF step: 1: 2: 3: 4: 5: 6: Voltage (V) 150: 300: 600: 1500: 3000: 300: Time (H) 0.5: 0.5: 0.5: 0.5: 0.5: 0.5: Volt-Hours: 75: 150: 300: 750: 7500-18 cm IPG strip. IEF step: 1: 2: 3: 4: 5: 6: Voltage (V) 300: 600: 1500: 3000: 300: Time (H) 0.5: 1: 1: 12: 20: Volt-Hours: 150: 600: 1500: 36000-
https://thebiotechnotes.com/2017/02/22/isoelectric-focusing-first-dimension/
During NEPHGE, the IEF gels are not actually “focused” to their pIs. The separation is based on the migration rates of the differentially charged polypeptides as they move across the gel. Therefore, it is necessary to pay strict attention to accumulated volt hours during the run to assure reproducible patterns in subsequent separations.
http://www.americanlaboratory.com/913-Technical-Articles/18747-A-Device-for-Isoelectric-Focusing-of-IPG-Gel-Strips/
Volt-hour focusing values of 100,000 Vhrs are not uncommon, and newer IEF instruments utilize very high voltages to speed focusing times. During the final focusing steps of the IEF separation, a higher voltage applied will accumulate the required Vhrs in a shorter time period, something easily accomplished with the Hoefer IEF100.
https://consort.be/wp-content/uploads/man_eief1100.pdf
Volt-hours 75 150 300 750 7500 - Recommended 150 Running Conditions for IEF of 18cm SERVA IPG strips Voltage Step 1 2 3 4 5 end of run Voltage (V) 300 600 0 3000 330 Time (h) 1 1 1 12.5 <20 Volt-hours 300 600 150 0 37500 - 6.3 Installation and Running Precast or Hand-cast Gels
https://www.cytivalifesciences.com/en/us/shop/protein-analysis/electrophoresis-and-isoelectric-focusing/protein-electrophoresis-equipment/ettan-ipgphor-3-ief-system-p-05529
Ettan™ IPGphor™ 3 IEF system is a fully integrated isoelectric focusing system optimized to deliver speed and reliability in the first dimension of 2-D electrophoresis. Run up to 12 IPG strips (7, 11, 13, 18, or 24 cm) simultaneously using Ettan™ IPGphor™ manifold, Ettan™ IPGphor™ Manifold Light, or ceramic strip holders.
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