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https://www.intechopen.com/books/novel-gene-therapy-approaches/extracellular-and-intracellular-barriers-to-non-viral-gene-transfer
To maximize non-viral gene delivery, levels of expression must be improved. Unfortunately, many extracellular and intracellular barriers (including the extracellular environment, immune scavengers, the cell membrane, endosomal escape, the cytoskeletal network and the nuclear membrane) preclude efficient gene transfer.Author: Lynn F. Gottfried, David A. Dean
https://www.researchgate.net/publication/11269723_Intracellular_Barriers_to_Non-Viral_Gene_Transfer
Intracellular Barriers to Non-Viral Gene Transfer Current Gene Therapy, 2002, Vol. 2, No. 2 7 Nuclear Delivery of Plasmid DNA The significant size of plasmid DNA (2-10 MDa) makes
https://www.ncbi.nlm.nih.gov/pubmed/12109215
1. Curr Gene Ther. 2002 May;2(2):183-94. Intracellular barriers to non-viral gene transfer. Lechardeur D(1), Lukacs GL. Author information: (1)Hospital for Sick Children, Program in Cell and Lung Biology, Department of Laboratory Medicine and Pathobiology, University of Toronto, 555 University Av., Toronto, Ontario, Canada, M5G 1X8. [email protected]: Delphine Lechardeur, Gergely L. Lukacs
https://www.researchgate.net/publication/8991580_Extracellular_and_intracellular_barriers_in_non-viral_gene_delivery_1
Low transfection efficiency of nonviral gene delivery vectors results mainly from multiple extracellular and intracellular barriers.1, 2 Among the intracellular barriers, endosomal escape has been ...
https://www.doctorabel.us/cell-therapy/iv-extracellular-barriers-for-gene-delivery.html
Sep 17, 2018 · In vitro gene delivery and ex vivo gene therapy approaches are predominantly concerned with gene delivery barriers presented by the cell itself. In vivo gene therapy must also be concerned with extracellular barriers, where the route of administration of the therapeutic gene also plays a …
https://www.sciencedirect.com/science/article/pii/S1748013218300793
Biological barriers for non-viral gene therapy are typically divided into two categories: extracellular (EC) and intracellular (IC) barriers. In short, EC barriers need to be overcome prior to reaching the target cell. In Fig. 1, the barriers after systemic administration are shown. These include endo- and exonuclease activity in the blood ...Author: Lotte M.P. Vermeulen, Toon Brans, Stefaan C. De Smedt, Katrien Remaut, Kevin Braeckmans
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006671/
2.2 Intracellular Barriers. It has been reported that although >95% of cells in culture typically internalize vectors, only a small fraction, typically <50%, express the transgene ().Following internalization, gene delivery vectors are challenged by intracellular barriers, including endosome entrapment, lysosomal degradation, nucleic acid unpacking from vectors, translocation across the ...Author: Yi Zhao, Leaf Huang
https://pubs.acs.org/doi/10.1021/mp400467x
Oct 04, 2013 · Methodologies to investigate intracellular barriers for nucleic acid delivery in non-viral gene therapy. Nano Today 2018 , 21 , 74-90. DOI: 10.1016/j.nantod.2018.06.007.Author: Charles H. Jones, Chih-Kuang Chen, Anitha Ravikrishnan, Snehal Rane, Blaine A. Pfeifer
https://www.sciencedirect.com/science/article/pii/S0168365903003973
Extracellular and intracellular barriers in non-viral gene delivery ... Non-viral gene delivery systems are, however, less efficient than the viral systems. Cationic liposomes (lipoplexes) and cationic polymers are the most frequently used non-viral gene transfer systems. Once in contact with negatively charged DNA, these lipids and polymers ...Author: Marika Ruponen, Paavo Honkakoski, Seppo Rönkkö, Jukka Pelkonen, Markku Tammi, Arto Urtti
https://cdn.intechopen.com/pdfs/42454/InTech-Extracellular_and_intracellular_barriers_to_non_viral_gene_transfer.pdf
mice and rats [28-37]. Finally, hydrodynamic gene delivery is a highly efficient method for gene transfer to highly perfused organs such as the liver or muscle in peripheral limbs. A large volume of DNA injected into the tail vein of a mouse has been shown to result in transient Extracellular and Intracellular Barriers to Non-Viral Gene Transfer
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