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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3900005/
Jan 01, 2014 · In summary, in the 23 years since our initial demonstration of the effectiveness of in vivo gene transfer using recombinant replication-deficient adenovirus vectors, together with adeno-associated viruses and lenti/retrovirus vectors, the adenovirus now represents one of the three major viral vector categories in the gene therapy tool kit. Through studies with adenovirus vectors, we …Author: Ronald G. Crystal
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3392980/
The promise of cancer gene therapeutics is hampered by difficulties in the in vivo delivery to the targeted tumor cells, and systemic delivery remains to be the biggest challenge to be overcome. Here, we concentrate on systemic in vivo gene delivery for cancer therapy using nonviral vectors. In this review, we summarize the existing delivery barriers together with the requirements and strategies to …Author: Yuan Zhang, Andrew Satterlee, Leaf Huang
https://www.nature.com/articles/3301043
Jan 05, 2000 · To test cell type-specific gene delivery in vivo, a mouse model system has been developed. Antibiotic resistant human target and non-target cells …Author: A Jiang, R Dornburg
https://www.liebertpub.com/doi/full/10.1089/hum.2013.2527
Jan 20, 2014 · FIG. 1. Schematic of a typical adenovirus gene transfer vector genome. In an adenovirus vector, the early (E) genes in the E1 region are deleted (to prevent replication) as is the E3 region (to make more room for the expression cassette).Author: G CrystalRonald
https://seekingalpha.com/article/4305556-gene-therapy-series-2-delivery-systems-in-gene-therapy-including-viral-vectors
Nov 11, 2019 · AAV-based transgene delivery is the most common form of in vivo gene delivery. In the ex vivo delivery, lentivirus vectors dominate the delivery approaches. In …
http://www.biologydiscussion.com/gene/therapy/gene-therapy-ex-vivo-and-in-vivo-gene-therapy-with-diagram/9969
The direct delivery of genes into the cells of a particular tissue is referred to as in vivo gene therapy. The ex vivo gene therapy can be applied to only selected tissues (e.g., bone marrow) whose cells can be cultured in the laboratory. The technique of ex vivo gene therapy …
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