We have collected information about In Vitro Sirna Delivery for you. Follow the links to find out details on In Vitro Sirna Delivery.
http://altogenlabs.com/rna-interference-rnai-services/in-vivo-sirna-delivery-and-tissue-targeting/
In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi) In Vivo siRNA Delivery and Tissue Targeting. RNAi-induced gene silencing is used to study gene function in cultured cells (in vitro).Most often, these effects are induced by the introduction of small-interfering RNA (siRNA) or microRNA (miRNA).
https://www.nature.com/articles/nprot.2012.131
Nov 15, 2012 · Exosomes are promising tools for drug delivery across different biological barriers. Here we show how exosomes derived from cultured cells can be harnessed for delivery of siRNA in vitro …Author: Samir El-Andaloussi, Samir El-Andaloussi, Yi Lee, Samira Lakhal-Littleton, Jinghuan Li, Yiqi Seow, C...
https://www.sciencedirect.com/science/article/pii/S0927775718305880
Oct 20, 2018 · For in vitro siRNA delivery assays, a plasmid vector coding for GL3 luciferase (pVAX1-LUC) under the control of the CMV (cytomegalovirus) promoter was used. The plasmid DNA was purified by using a PureLink HiPure Plasmid Filter Maxiprep Kit (Invitrogen, Thermo Fisher Scientific), and its concentration was determined by reading the absorbance at ...Author: Ismail Eş, Meryem Tyrrasch Ok, Ximena E. Puentes-Martinez, Marcelo Augusto Szymanski de Toledo, Mari...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995415/
May 05, 2010 · To improve the evaluation of siRNA delivery in vivo, we tailored an approach that quantifies the small RNAs residing in the RISC from the tissues of rodents and monkey. Application of our approach to preclinical animal studies yielded quantitative insights into the in vivo efficiency of siRNA delivery and the mechanism of RNA silencing.Author: Yi Pei, Paula J. Hancock, Hangchun Zhang, René Bartz, Craig Cherrin, Nathalie Innocent, Colin J. Pom...
https://www.sciencedirect.com/science/article/pii/S0168365918300774
Herein we present the use of the VIPER polymer system for the encapsulation and delivery of siRNA in vitro and in vivo. Our present work demonstrates the impressive ability of the VIPER system to condense and protect siRNA at low N/P ratios while physical characterization revealed uniform particles with >200 nm hydrodynamic diameters.Author: Daniel P. Feldmann, Yilong Cheng, Rima Kandil, Yuran Xie, Mariam Mohammadi, Hartmann Harz, Akhil Sha...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207430/
To confirm endothelial localization resulted in functional siRNA delivery, gene silencing was measured after 7C1 was formulated with siRNAs targeting genes expressed primarily by endothelial cells. 7C1 was formulated with siRNA targeting the gene ICAM-2 and injected with a …Author: James E. Dahlman, Carmen Barnes, Omar F. Khan, Aude Thiriot, Siddharth Jhunjunwala, Taylor E. Shaw, ...
http://www.invivotransfection.com/
In Vivo Transfection In Vivo Background . With modern genetic techniques allowing scientists to identify the link between genes and diseases, there is an enormous emphasis on determining ways to alter the function of a gene in vivo.Strategies to treat these diseases include delivering an alternate gene to the cell, replacing the defective gene or silencing the defective gene using RNAi or ...
https://www.thermofisher.com/us/en/home/references/ambion-tech-support/rnai-sirna/tech-notes/high-throughput-delivery-of-sirnas-examples.html
In the article, High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells, we examined the efficiency and reproducibility of two techniques for high throughput delivery of siRNAs: reverse transfection and 96 well electroporation.
Searching for In Vitro Sirna Delivery?
You can just click the links above. The data is collected for you.
