Hydrodynamics Based Gene Delivery

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Hydrodynamic Gene Delivery and Its Applications in ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064722/
    6. Hydrodynamic delivery for gene drug discovery. The advantage for the use of hydrodynamic gene delivery to study therapeutic functions of a gene is obvious. A candidate gene in plasmid or DNA fragment with regulatory sequence for its expression can be hydrodynamically injected into an animal (mouse or rat) carrying a target disease.Author: Barbara Bonamassa, Li Hai, Dexi Liu

Hydrodynamic Gene Delivery: Its Principles and Applications

    https://www.sciencedirect.com/science/article/pii/S1525001616327198
    Hydrodynamic gene delivery has been shown to be able to promote both humoral- and cell-based immunity in animals in a gene dependent manner. Hydrodynamic gene delivery generates much more gene product than does the commonly used method of intramuscle injection of plasmid DNA.Author: Takeshi Suda, Dexi Liu

Effect of Hydrodynamics-Based Gene Delivery of Plasmid DNA ...

    https://www.ahajournals.org/doi/10.1161/01.CIR.0000160348.75918.CA
    Background— Interleukin-1 (IL-1) is a powerful and important cytokine in myocarditis. The purpose of this study was to evaluate the effect and possible mechanism of hydrodynamics-based delivery of the IL-1 receptor antagonist (IL-1RA)-immunoglobulin (Ig) gene for treatment of rat experimental autoimmune myocarditis (EAM).Author: Hui Liu, Haruo Hanawa, Tsuyoshi Yoshida, Raafat Elnaggar, Manabu Hayashi, Ritsuo Watanabe, Ken Toba,...

Hydrodynamic Delivery - ScienceDirect

    https://www.sciencedirect.com/science/article/pii/S0065266005540045
    Hydrodynamic delivery has emerged as a near‐perfect method for intracellular DNA delivery in vivo.For gene delivery to parenchymal cells, only essential DNA sequences need to be injected via a selected blood vessel, eliminating safety concerns associated with current viral and synthetic vectors.

Improvement of Hydrodynamics-Based Gene Transfer of ...

    https://www.hindawi.com/journals/bmri/2013/928790/
    In this study, we examined the effect of various types of gene delivery carriers on enhancing the uptake and gene expression of exogenous DNA in murine hepatocytes when a hydrodynamics-based gene delivery (HGD) is performed via tail-vein injection.Author: Shingo Nakamura, Tadaaki Maehara, Satoshi Watanabe, Masayuki Ishihara, Masahiro Sato

Image-Guided Hydrodynamic Gene Delivery: Current Status ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588196/
    Aug 21, 2015 · Hydrodynamics-based delivery has been used as an experimental tool to express transgene in small animals. This in vivo gene transfer method is useful for functional analysis of genetic elements, therapeutic effect of oligonucleotides, and cancer cells to establish the metastatic cancer animal model for experimental research. Recent progress in the development of image-guided …Author: Kenya Kamimura, Takeshi Yokoo, Hiroyuki Abe, Yuji Kobayashi, Kohei Ogawa, Yoko Shinagawa, Ryosuke In...

Hydrodynamics-based transfection in animals ... - Gene Therapy

    https://www.nature.com/articles/3300947
    Aug 09, 1999 · Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA. ... compared with other vector-mediated gene delivery …Author: F Liu, Y K Song, D Liu

Hydrodynamics-based delivery of the viral ... - Gene Therapy

    https://www.nature.com/articles/3301988
    Jul 28, 2008 · This is known as ‘hydrodynamics-based transfection′. 20,21 We demonstrated that this technique is also useful for the delivery of a therapeutic protein into normal rats, using a rat ...Author: N Higuchi, H Maruyama, T Kuroda, S Kameda, N Iino, H Kawachi, Y Nishikawa, H Hanawa, H Tahara, J Miy...

Effect of Hydrodynamics-Based Gene Delivery of Plasmid DNA ...

    https://www.researchgate.net/publication/7941287_Effect_of_Hydrodynamics-Based_Gene_Delivery_of_Plasmid_DNA_Encoding_Interleukin-1_Receptor_Antagonist-Ig_for_Treatment_of_Rat_Autoimmune_Myocarditis_Possible_Mechanism_for_Lymphocytes_and_Noncardiac_C
    Effect of Hydrodynamics-Based Gene Delivery of Plasmid DNA Encoding Interleukin-1 Receptor Antagonist-Ig for Treatment of Rat Autoimmune Myocarditis Possible Mechanism for Lymphocytes and ...

The effect of hydrodynamics‐based delivery of an IL‐13‐Ig ...

    https://onlinelibrary.wiley.com/doi/full/10.1002/eji.200425776
    Hydrodynamics‐based gene delivery can transfer into hepatocytes by retrograde blood flow from hepatic veins. Hydrodynamics‐based transfection is more efficient for the production of a therapeutic protein than gene transfer into muscle by electroporation in vivo, because the former method allows greater retention of synthesized proteins in ...Author: Raafat Elnaggar, Haruo Hanawa, Hui Liu, Tsuyoshi Yoshida, Manabu Hayashi, Ritsuo Watanabe, Satoru Ab...

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