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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722627/
Alternative gene therapy . Another means of using bacteria for gene therapy is the so-called Alternative gene therapy (AGT) approach, which is also known as bacterial protein delivery. It is based on the transfer of bacterially expressed therapeutic proteins to the host organism using genetically modified (transformed) bacteria.
https://seekingalpha.com/article/4305556-gene-therapy-series-2-delivery-systems-in-gene-therapy-including-viral-vectors
Nov 11, 2019 · Examples of gene therapy methods using ex vivo delivery include CAR-T therapies like Yescarta and Kymriah. In vivo delivery: By definition, this …
https://www.sciencedirect.com/science/article/pii/S0378517313010351
Gene therapy is a promising new technique for treating many serious incurable diseases, such as cancer and genetic disorders. The main problem limiting the application of this strategy in vivo is the difficulty of transporting large, fragile and negatively charged molecules like DNA into the nucleus of the cell without degradation.Author: D. Ibraheem, A. Elaissari, H. Fessi
https://parkinsonsnewstoday.com/2019/12/18/new-gene-therapy-delivery-method-brave-new-world-parkinsons/
Dec 18, 2019 · In gene therapy, scientists deliver a working version of a faulty gene using a harmless AAV that was modified and inactivated in the lab. This way the virus functions only as a delivery vehicle and does not have the capacity to damage tissues and cause disease.
https://www.medicinenet.com/gene_therapy_-_the_future_is_here/views.htm
The selection of a gene delivery system depends on the target cell, the duration of gene expression required for therapeutic effect, and the size of the piece of DNA to be used in the gene therapy. Genes can be carried into cells by viruses .
https://www.cell.com/molecular-therapy-family/methods/fulltext/S2329-0501(16)30165-6
Jan 01, 2016 · Gene therapy is a promising strategy for specific treatment of numerous gene-associated human diseases by intentionally altering the gene expression in pathological cells. A successful clinical application of gene-based therapy depends on an efficient gene delivery system.Author: Jie Chen, Zhaopei Guo, Huayu Tian, Xuesi Chen
https://ghr.nlm.nih.gov/primer/therapy/procedures
Oct 29, 2019 · Gene therapy is designed to introduce genetic material into cells to compensate for abnormal genes or to make a beneficial protein. If a mutated gene causes a necessary protein to be faulty or missing, gene therapy may be able to introduce a normal copy of the gene to restore the function of the protein. A gene...
http://www.biologydiscussion.com/gene/therapy/gene-therapy-ex-vivo-and-in-vivo-gene-therapy-with-diagram/9969
The direct delivery of genes into the cells of a particular tissue is referred to as in vivo gene therapy. The ex vivo gene therapy can be applied to only selected tissues (e.g., bone marrow) whose cells can be cultured in the laboratory. The technique of ex vivo gene therapy involves the following steps (Fig. 13.2).
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