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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722627/
Alternative gene therapy . Another means of using bacteria for gene therapy is the so-called Alternative gene therapy (AGT) approach, which is also known as bacterial protein delivery. It is based on the transfer of bacterially expressed therapeutic proteins to the host organism using genetically modified (transformed) bacteria.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · Physical methods of nonviral gene delivery. Physical methods applied for in vitro and in vivo gene delivery are based on making transient penetration in cell membrane by mechanical, electrical, ultrasonic, hydrodynamic, or laser-based energy so that DNA entrance into the targeted cells is facilitated.
https://www.slideshare.net/Aparajitha_Anne/gene-delivery-system
May 19, 2012 · Conclusion:• Gene therapy utilizes the delivery of DNA into cells, which can be done by several ways by a number of methods that is some of them are by viral methods and some are by the non-viral methods few are by the Hybrid methods.•
https://www.sciencedirect.com/topics/neuroscience/gene-delivery
Gene delivery using nonviral methods is an exciting research area that has been explored for intracellular transgene expression in gene therapy. Nonviral gene delivery is achieved using both physical and chemical methods for safe and efficient transgene expression in target cells.
https://seekingalpha.com/article/4305556-gene-therapy-series-2-delivery-systems-in-gene-therapy-including-viral-vectors
Nov 11, 2019 · Examples of gene therapy methods using ex vivo delivery include CAR-T therapies like Yescarta and Kymriah. In vivo delivery: By definition, this means inside the body. In …
https://www.sciencedirect.com/science/article/pii/S0378517313010351
Gene therapy is a promising new technique for treating many serious incurable diseases, such as cancer and genetic disorders. The main problem limiting the application of this strategy in vivo is the difficulty of transporting large, fragile and negatively charged molecules like DNA into the nucleus of the cell without degradation. The key to success of gene therapy is to create safe and ...Author: D. Ibraheem, A. Elaissari, H. Fessi
https://hemophilianewstoday.com/2018/04/02/new-delivery-method-may-improve-targeted-therapies-hemophilia-others-study/
Apr 02, 2018 · A delivery method using nanotubes with a spear-like shape may open new avenues for safer, faster, and more cost-effective gene therapies for several genetic diseases, including hemophilia, according to a study. The study, “Precision-Guided Nanospears for Targeted and High-Throughput...
https://www.intechopen.com/books/recent-advances-in-novel-drug-carrier-systems/gene-delivery-systems-recent-progress-in-viral-and-non-viral-therapy
Conversely, in acquired diseases, a single gene cannot be defined as the sole cause of a disease. Gene therapy has increased in prominence, and has shown great potential in treating acquired and genetic diseases (Conwell and Huang, 2005). Gene delivery systems are categorized as: viral-based, non-viral-based and combined hybrid systems.Author: Erdal Cevher, Ali Demir Sezer, Emre Şefik Çağlar
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