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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811526/
iPS cell technology is still in its infancy. In this review article, we present a comprehensive survey of reprogramming approaches focusing on gene-delivery systems used for generation of iPS cells from somatic cells, categorize gene-delivery vectors, and discuss their advantages and limitations for somatic cell reprogramming.Author: Lijian Shao, Wen Shu Wu
https://www.researchgate.net/publication/41101123_Gene-delivery_systems_for_iPS_cell_generation
In this review article, we present a comprehensive survey of reprogramming approaches focusing on gene-delivery systems used for generation of iPS cells from somatic cells, categorize gene ...
http://europepmc.org/abstract/MED/20088717
Feb 01, 2010 · iPS cell technology is still in its infancy. In this review article, we present a comprehensive survey of reprogramming approaches focusing on gene-delivery systems used for generation of iPS cells from somatic cells, categorize gene-delivery vectors, and discuss their advantages and limitations for somatic cell reprogramming.
https://link.springer.com/chapter/10.1007/978-981-10-1560-1_11
Dec 10, 2016 · Each gene delivery and expression system has particular advantages and disadvantages depending on the desired outcome of the experimental design. Here, we briefly review representative gene delivery and expression systems from the perspective of their application to iPS cell research.Author: Maolin Zhang, Kunimichi Niibe, Takeru Kondo, Yuya Kamano, Makio Saeki, Hiroshi Egusa
https://www.researchgate.net/publication/311556058_Gene_Delivery_and_Expression_Systems_in_Induced_Pluripotent_Stem_Cells
Gene Delivery and Expression Systems in Induced Pluripotent Stem Cells. ... Gene Delivery and Expression Systems . ... during reprogramming for iPS cell generation because it is necessary for the ...
https://www.sciencedirect.com/science/article/pii/S174067490800005X
Here, we assess the benefits and potential disadvantages of commonly used gene delivery systems for application to stem cell biology and iPS cell reprogramming. Gene delivery systems are varied (see Table 1) and substantial and the breadth of viral and non-viral technologies has developed at a great pace during the past 15 years within the gene ...Author: Nicole M. Kane, Scott McRae, Chris Denning, Andrew H. Baker
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561994/
The discovery of direct cell reprogramming and induced pluripotent stem (iPS) cell technology opened up new avenues for the application of non-viral, transposon-based gene delivery systems. The Sleeping Beauty (SB) transposon is highly advanced for versatile genetic manipulations in mammalian cells.Author: Ivana Grabundzija, Jichang Wang, Attila Sebe, Attila Sebe, Zsuzsanna Erdei, Robert Kajdi, Anantharam...
https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(18)30595-1
Apr 10, 2019 · Generation of iPSCs with gene delivery systems that do not integrate and are not self-replicating requires several cycles of transfection with exogenous reprogramming factors until endogenous transcription factors reach sufficiently high protein levels and take over.Author: Alexandra G. Orlandini von Niessen, Marco A. Poleganov, Corina Rechner, Arianne Plaschke, Lena M. Kr...
https://www.sciencedirect.com/science/article/pii/S0925443919300432
An elastin-like polypeptide enabled non-viral gene delivery into cells in vitro. • Mouse embryonic fibroblasts were reprogrammed into induced pluripotent stem cells. • Process was reproducible and multiple iPS cell lines were generated. • All iPS cells had stem cell-like markers and differentiated into the three germ layers. •Author: Chang Hyun Lee, Rohan S.J. Ingrole, Harvinder Singh Gill
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