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https://www.researchgate.net/publication/11060999_Evaluation_of_Strategies_for_the_Intracellular_Delivery_of_Proteins
In this study we utilize a sensitive luciferase reporter gene assay to provide unambiguous and quantitative evaluation of several strategies for the intracellular delivery of a biologically active ...
https://www.ncbi.nlm.nih.gov/pubmed/12403066
Evaluation of strategies for the intracellular delivery of proteins. Ye D(1), Xu D, Singer AU, Juliano RL. Author information: (1)Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, 27599-7365, USA.Author: Dongjiu Ye, Dong Xu, Alex U. Singer, R. L. Juliano
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.399.7428
The intracellular delivery of functionally active proteins represents an important emerging strategy for laboratory investigation and therapeutic applications. Although a number of promising approaches for protein delivery have been developed, thus far there has been no attempt to compare the merits of the various delivery technologies.
https://link.springer.com/article/10.1023%2FA%3A1020346607764
The intracellular delivery of functionally active proteins represents an important emerging strategy for laboratory investigation and therapeutic applications. Although a number of promising approaches for protein delivery have been developed, thus far there has been no attempt to compare the merits of the various delivery technologies.Author: Dongjiu Ye, Dong Xu, Alex U. Singer, R. L. Juliano
https://pubs.acs.org/doi/10.1021/acs.chemrev.7b00678
Intracellular delivery is a key step in biological research and has enabled decades of biomedical discoveries. It is also becoming increasingly important in industrial and medical applications ranging from biomanufacture to cell-based therapies. Here, we review techniques for membrane disruption-based intracellular delivery from 1911 until the present. These methods achieve rapid, direct, and ...Author: Martin P. Stewart, Martin P. Stewart, Robert Langer, Klavs F. Jensen
https://www.sciencedirect.com/science/article/pii/S0958166917302185
Another strategy for the intracellular delivery of TFs is to tether proteins to protein transduction domains (PTDs) such as TAT, ANTP and VP22, which can potentially cross biological membranes efficiently . For example, a fusion protein composed of the TAT peptide fused with a zinc finger protein targeting the SV40 promoter, and the KRAB ...Author: Yumiao Zhang, Joachim Justad Røise, Kunwoo Lee, Jie Li, Niren Murthy
https://pubs.acs.org/doi/10.1021/jacs.8b12705
Protein delivery to the cytosol is challenging due to the high mol. wt. and the polarity of therapeutic proteins. Here we review strategies for the delivery of proteins into cells, including cell-penetrating peptides, virus-like particles, supercharged proteins, nanocarriers, polymers, …Author: Shunzhi Wang, Yijing Chen, Shuya Wang, Peng Li, Chad A. Mirkin, Omar K. Farha
https://www.sciencedirect.com/science/article/pii/S2162253116300294
In this study, we demonstrate rapid and efficient delivery of catalytic protein and antibodies into the cytosol of cultures cells using liposome-based formulation. Intracellular delivery of functional proteins provides a potential tool to replace missing, dysfunctional and poorly expressed proteins or antagonize key intracellular pathways.
https://core.ac.uk/display/23684432
Purpose. The intracellular delivery of functionally active proteins represents an important emerging strategy for laboratory investigation and therapeutic applications. Although a number of promising approaches for protein delivery have been developed, thus far there has been no attempt to compare the merits of the various delivery technologies.
https://www.sciencedirect.com/science/article/pii/S1359644617302234
The intracellular delivery of nanomaterials and drugs has been attracting increasing research interest, mainly because of their important effects and functions in several organelles. Targeting specific organelles can help treat or decrease the symptoms of diabetes, cancer, …
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