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https://www.nature.com/articles/s41598-018-38119-9
Feb 15, 2019 · A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall Abstract. Genome engineering in plants is highly dependent on the availability... Introduction. Genome engineering is a …Author: Yuichi Furuhata, Ayako Sakai, Tomi Murakami, Mone Morikawa, Mone Morikawa, Chikashi Nakamura, Chikas...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4078794/
Electroporation-mediated delivery of molecules is a procedure widely used for transfecting complementary DNA in bacteria, mammalian and plant cells. This technique has proven very efficient for the introduction of macromolecules into cells in suspension culture and even into cells in their native tissue environment, e.g. retina and embryonic tissues.Author: Ami A. Deora, Fernando Diaz, Ryan Schreiner, Enrique Rodriguez-Boulan
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463957/
Jun 11, 2015 · After electroporation, the eggs were immediately collected from the electrode chamber and subjected to four washes with M2 medium followed by two washes with mWM medium. The eggs were then cultured in mWM medium at 37 °C and 5% CO2 incstage.Author: Masakazu Hashimoto, Masakazu Hashimoto, Tatsuya Takemoto
https://www.intechopen.com/books/application-of-nanotechnology-in-drug-delivery/electroporation-advantages-and-drawbacks-for-delivery-of-drug-gene-and-vaccine
Electroporation has proven useful both in vitro, in vivo and in patients, where drug delivery to malignant tumors has been performed. In addition, the data show that electroporation of DNA vaccines in vivo is an effective method to increase cellular uptake of DNA and gene expression in tissue leading to marked improvement in immune responses.Author: Azam Bolhassani, Afshin Khavari, Zahra Orafa
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3516926/
Apr 10, 2012 · The potential advantages of microneedle-based delivery include 1) local administration of DNA into skin with minimal pain, 2) facilitation of DNA uptake locally within the skin, and 3) expected good patient acceptance. Ideally, electroporation would cause molecular uptake/transfection in all cells with no loss of viability.Author: Seong-O Choi, Yeu-Chun Kim, Yeu-Chun Kim, Jeong Woo Lee, Jung-Hwan Park, Mark R. Prausnitz, Mark G. ...
https://www.portlandpress.com/biochemsoctrans/article/doi/10.1042/BST20190039/222602/Methods-for-protein-delivery-into-cells-from
The manipulation of cultured mammalian cells by the delivery of exogenous macromolecules is one of the cornerstones of experimental cell biology. Although the transfection of cells with DNA expressions constructs that encode proteins is routine and simple to perform, the direct delivery of proteins into cells has many advantages. For example, proteins can be chemically modified, assembled into ...
https://www.maxcyte.com/technology/flow-electroporation/
Flow Electroporation® technology is based on a fundamental principle of cell membranes – the reversible permeability of membranes in the presence of an electrical field – thereby creating a universal transfection technology capable of high-performance delivery of virtually any molecule(s), including DNA, RNA, proteins and cell lysates, to any cell type with minimal cell disturbance.
https://www.sciencedirect.com/science/article/pii/S1673852716300029
Delivery of Cas9 protein through electroporation achieved high efficiency of genome deletion in an inbred strain. A: Schematic of the strategy to delete DNA fragment from the Smc1b gene locus. A DNA segment including exon 2, exon 3 and exon 4 was deleted from the genome.Author: Wenbo Wang, Peter M. Kutny, Shannon L. Byers, Charles J. Longstaff, Michael J. DaCosta, Changhong Pa...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151968/
Oct 16, 2007 · Protein delivery procedure. Cells were plated in order to reach approximately 70–80% confluency the day of protein delivery experiment. For one well of a 24-well plate, 0.5–8 μg of purified protein was diluted in 100 μL of Hepes buffer (20 mM, pH 7.4) in a 1.5 mL microcentrifuge tube, under sterile conditions.Author: Claire O. Weill, Stéphanie Biri, Abdennaji Adib, Patrick Erbacher
https://www.sciencedirect.com/science/article/pii/S016816561500200X
Upon association of gRNA with purified Cas9 protein, the Cas9 RNPs are used to transfect cells via lipid-mediated delivery or electroporation. As early as day 3 (24 h post transfection), the cells can be harvested for analysis of locus-specific genome modification efficiency.Author: Xiquan Liang, Jason Potter, Shantanu Kumar, Yanfei Zou, Rene Quintanilla, Mahalakshmi Sridharan, Jas...
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