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https://www.ncbi.nlm.nih.gov/pubmed/21078116
Enhanced specificity and efficiency of RNAi in vivo via selective accumulations in desired tissues, specific binding to target cells and facilitated intracellular trafficking are also commonly attempted utilizing targeting moieties, cell-penetrating peptides, fusogenic peptides and stimuli-responsive polymers.Author: Min Suk Shim, Young Jik Kwon
https://febs.onlinelibrary.wiley.com/doi/full/10.1111/j.1742-4658.2010.07904.x
Oct 11, 2010 · Targeted siRNA delivery in vivo. In order to achieve RNAi in vivo via systemic delivery, it is crucial for siRNA to be efficiently located in desired tissues/cells. This requires three important processes: prolong circulation in the body, high accessibility to target tissues and specific binding to target cells.Author: Min Suk Shim, Young Jik Kwon
http://onlinelibrary.wiley.com/doi/10.1111/j.1742-4658.2010.07904.x/abstract
This minireview introduces major challenges in achieving efficient siRNA delivery in vivo and discusses recent advances in overcoming them using chemically modified siRNA, viral siRNA vectors and nonviral siRNA carriers.
https://febs.onlinelibrary.wiley.com/doi/abs/10.1111/j.1742-4658.2010.07904.x
Efficient and targeted delivery of siRNA in vivo Min Suk Shim Department of Chemical Engineering and Materials Science, University of California, Irvine, CA, USAAuthor: Min Suk Shim, Young Jik Kwon
https://www.researchgate.net/publication/47793471_Efficient_and_targeted_delivery_of_siRNA_in_vivo
Request PDF Efficient and targeted delivery of siRNA in vivo RNA interference (RNAi) has been regarded as a revolutionary tool for manipulating target biological processes as well as an ...
https://www.academia.edu/20664200/Efficient_and_targeted_delivery_of_siRNA_in_vivo
Unlike viral vectors, which deliver siRNA in the form of a viral genome, nonviral carriers deliver native Fig. 2. An ideally designed nonviral siRNA carrier for efficient and siRNA, generate low immunogenicity and offer high targeted RNAi in vivo…
https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1742-4658.2010.07904.x%4010.1002/%28ISSN%291742-4658%28CAT%29FreeReviewContent%28VI%29Reviews1011
in vivo delivery systems is pivotal in overcoming the challenges in achieving efficient and targeted siRNA delivery in vivo. Major hurdles in siRNA delivery in vivo and various approaches to overcoming them are illustrated in Fig. 1. Local versus systemic delivery …Author: Min Suk Shim, Young Jik Kwon
http://altogenlabs.com/rna-interference-rnai-services/in-vivo-sirna-delivery-and-tissue-targeting/
In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi) In Vivo siRNA Delivery and Tissue Targeting. RNAi-induced gene silencing is used to study gene function in cultured cells (in vitro). Most often, these effects are induced by the introduction of small-interfering RNA (siRNA…
https://www.nature.com/articles/nprot.2012.131
Nov 15, 2012 · Here we show how exosomes derived from cultured cells can be harnessed for delivery of siRNA in vitro and in vivo. This protocol first describes the generation of targeted …Author: Samir El-Andaloussi, Samir El-Andaloussi, Yi Lee, Samira Lakhal-Littleton, Jinghuan Li, Yiqi Seow, C...
https://altogen.com/transfection-resource/in-vivo-sirna-transfection/
In Vivo siRNA Transfection. In Vivo siRNA Transfection (animal siRNA delivery) In vivo methods require live animal systems where the desired molecule is delivered into the animal or locally to specific tissues. The immune response of the animal will influence the transfection efficiency. By targeting …
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