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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6859480/
SIGS vs HIGS: a study on the efficacy of two dsRNA delivery strategies to silence Fusarium FgCYP51 genes in infected host and non‐host plants. Aline Koch, 1 , † Lisa Höfle, 1 , † Bernhard Timo Werner, 1 Jafargholi Imani, 1 Alexandra Schmidt, 1 Lukas Jelonek, 2 and Karl‐Heinz Kogel 1Author: Aline Koch, Lisa Höfle, Bernhard Timo Werner, Jafargholi Imani, Alexandra Schmidt, Lukas Jelonek, Ka...
https://www.sciencedirect.com/science/article/pii/S2095311913605110
However, one of the key limitations in pest control is lack of the convenient and efficient method for dsRNA delivery. In this study, different dsRNA delivery methods at their own optimum conditions were evaluated comparatively for their efficiency with Helicoverpa armigera as test animal. It was found that the popular one-time injection of ...Author: Jing Yang, Zhao-jun Han
http://onlinelibrary.wiley.com/doi/10.1111/j.1744-7917.2012.01534.x/abstract
This review focuses on the approaches of introducing dsRNA into insect cells or insect bodies to induce effective RNAi. The three most common delivery methods, namely, microinjection, ingestion, and soaking, are illustrated in details and their advantages and limitations are summarized for purpose of feasible RNAi research.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2977003/
Jun 11, 2010 · The site of action of siRNA therapeutics is the cytosol. The barriers to siRNA delivery are multiple and depend on the targeted organs and the administration routes. In general, locoregional delivery of siRNA has fewer barriers compared to systemic delivery.
https://www.frontiersin.org/articles/10.3389/fphys.2017.00256/full
Apr 28, 2017 · The strategy of oral dsRNA delivery involves the exposure of dsRNA to the gut lumen environment. Here, one crucial obstacle is the presence of gut nucleases. We speculated that the interaction of PTD-DRBD with dsRNA might offer protection against nuclease activity by covering the dsRNA.Author: François-Xavier Gillet, Rayssa A. Garcia, Leonardo L. P. Macedo, Erika V. S. Albuquerque, Maria C. M...
https://www.ncbi.nlm.nih.gov/pubmed/23955821
Delivery of dsRNA for RNAi in insects: an overview and future directions. Yu N(1), Christiaens O, Liu J, Niu J, Cappelle K, Caccia S, Huvenne H, Smagghe G. Author information: (1)Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, B …Author: Na Yu, Olivier Christiaens, Jisheng Liu, Jinzhi Niu, Kaat Cappelle, Silvia Caccia, Hanneke Huvenne, ...
https://www.frontiersin.org/articles/10.3389/fphys.2018.01912/full
Jan 11, 2019 · While viral delivery systems show a lot of potential and are generally considered to be among the most efficient methods for dsRNA delivery, their in vivo application has not been widely investigated yet (Kolliopoulou et al., 2017). This may be due to the many safety issues that accompany this method of delivery.Author: Elise Vogel, Dulce Santos, Lina Mingels, Thomas-Wolf Verdonckt, Jozef Vanden Broeck
https://www.sciencedirect.com/science/article/pii/S0048357519304596
Oct 22, 2019 · Successful delivery of dsRNA depends on the capacity of NPs to enter the cell, reach the cytoplasm, and then release the cargo . In most cases, NPs are internalized by an endocytosis and endo-lysosomal pathway. Therefore, endosomal escape is of particular importance for the delivery of dsRNA …Author: Kangxu Wang, Kangxu Wang, Yingchuan Peng, Jiasheng Jason Chen, Yue Peng, Xuesong Wang, Zihan Shen, Z...
https://onlinelibrary.wiley.com/doi/full/10.1002/arch.21645
Nov 19, 2019 · However, simply adding “naked” dsRNA to an artificial diet was an unreliable form of RNAi delivery in our hands due to dsRNA degradation. Delivery via host plants was more successful, and the new diet formulation was suitable for symbiont‐mediated dsRNA delivery, which we believe is the most convenient approach for large‐scale knockdown ...Author: Awawing A. Andongma, Carolyn Greig, Paul J. Dyson, Natasha Flynn, Natasha Flynn, Miranda M. A. Whitt...
https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12866
Oct 11, 2019 · Here we compare two different methods (setups) of dsRNA delivery, viz. transgene expression (host‐induced gene silencing, HIGS) and spray application (spray‐induced gene silencing, SIGS), to assess the activity of CYP3RNA and novel dsRNA species designed to target one or two FgCYP51 genes.Author: Aline Koch, Lisa Höfle, Bernhard Timo Werner, Jafargholi Imani, Alexandra Schmidt, Lukas Jelonek, Ka...
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