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https://www.sciencedirect.com/science/article/pii/S2095311913605110
However, one of the key limitations in pest control is lack of the convenient and efficient method for dsRNA delivery. In this study, different dsRNA delivery methods at their own optimum conditions were evaluated comparatively for their efficiency with Helicoverpa armigera as test animal. It was found that the popular one-time injection of ...Author: Jing Yang, Zhao-jun Han
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112363/
Nov 17, 2016 · The holistic understanding of systemic properties of dsRNA along with improvements toward delivery methods is underway, and in the coming years will provide innovative breakthrough applications for management of pest insects with a unique mode of action.Author: Mallikarjuna R. Joga, Moises J. Zotti, Guy Smagghe, Olivier Christiaens
https://www.ncbi.nlm.nih.gov/pubmed/29782023
May 04, 2018 · Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects. Ghosh SKB(1), Hunter WB(2), Park AL(1), Gundersen-Rindal DE(3). Author information: (1)Invasive Insect Biocontrol and Behavior Laboratory, Agricultural Research Service, United States Department of Agriculture.Author: Saikat Kumar B. Ghosh, Wayne B. Hunter, Alexis L. Park, Dawn E. Gundersen-Rindal
https://www.jove.com/video/57390/double-stranded-rna-oral-delivery-methods-to-induce-rna-interference
The clay complex can also be chemically configured to release the dsRNA under specific pH or ionic conditions. Clay delivery methods of dsRNA into plants have been shown to provide dsRNA into potted citrus trees, and other plants for over 14 months (data not shown).Author: Saikat Kumar B. Ghosh, Wayne B. Hunter, Alexis L. Park, Dawn E. Gundersen-Rindal
https://www.sciencedirect.com/science/article/abs/pii/S2095311913605110
In this study, different dsRNA delivery methods at their own optimum conditions were evaluated comparatively for their efficiency with Helicoverpa armigera as test animal. It was found that the popular one-time injection of larvae with dsRNA could reduce the pupation rate by 43.0% and enhance larva mortality by 11.7%. One-time ingestion of ...Author: Jing Yang, Zhao-jun Han
https://academic.oup.com/jee/article/112/1/434/5227427
Dec 03, 2018 · While injection of the dsRNA directly into the target insect hemocoel is likely the most efficient dsRNA delivery method to investigate phenotypic effects, it is not practical for the development of an RNAi-based control method.Author: Man-Yeon Choi, Robert K Vander Meer
http://onlinelibrary.wiley.com/doi/10.1111/j.1744-7917.2012.01534.x/abstract
This review focuses on the approaches of introducing dsRNA into insect cells or insect bodies to induce effective RNAi. The three most common delivery methods, namely, microinjection, ingestion, and soaking, are illustrated in details and their advantages and limitations are summarized for purpose of feasible RNAi research.
https://www.researchgate.net/publication/335338774_Spray_method_application_of_transdermal_dsRNA_delivery_system_for_efficient_gene_silencing_and_pest_control_on_soybean_aphid_Aphis_glycines
However, the existing dsRNA delivery methods largely restrict the application of the RNAi-based pest management strategy; thus, we previously constructed a nanocarrier-based transdermal dsRNA ...
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