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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679364/
In addition, viral vectors encoding shRNAs have shown promise for in vivo delivery; however, most of these studies have utilized adenoviral delivery of shRNA, which has well-known toxic effects in the animal . Recently, adeno-associated viral vectors (AAV) have been designed with less toxicity and adequate shRNA delivery .Author: Chris B. Moore, Elizabeth H. Guthrie, Max Tze-Han Huang, Debra J. Taxman
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2977003/
Jun 11, 2010 · Under in vivo conditions, siRNA and shRNA were equally effective in luciferase knockdown from 3 h to 3 days, although shRNA was significantly more potent on a molar basis . From the standpoint of transfection efficiency and delivery, siRNA poses advantages over shRNA.Author: Jie Wang, Ze Lu, M. Guillaume Wientjes, Jessie L.-S. Au
https://www.sciencedirect.com/science/article/pii/S0168165619301804
Evaluation of AAV-mediated delivery of shRNA to target basal-like breast cancer genetic vulnerabilities. Author links open overlay panel Catarina Pinto a b Gabriela Silva a Ana S. Ribeiro c d Mónica Oliveira c d Manuel Garrido a Vanessa S. Bandeira a Andr ...Author: Catarina Pinto, Gabriela Silva, Ana S. Ribeiro, Ana S. Ribeiro, Mónica Oliveira, Mónica Oliveira, Ma...
https://www.sciencedirect.com/science/article/pii/S0169409X09000969
Non-viral polymeric delivery systems, in particular those with biodegradable components, have much better safety profiles than their viral counterparts though their transfection efficiency is generally lower , , . Non-viral vehicles for delivery of siRNA and shRNA are typically cationic preparations.Author: Donald D. Rao, John S. Vorhies, Neil Senzer, John Nemunaitis
https://www.nature.com/articles/s41591-019-0674-1
Dec 23, 2019 · Injection of AAV–shRNA below the pial surface of the spinal cord prevents onset or ameliorates progression in a mouse model of ALS, and achieves widespread delivery …Author: Mariana Bravo-Hernandez, Takahiro Tadokoro, Takahiro Tadokoro, Michael R. Navarro, Oleksandr Platosh...
http://altogenlabs.com/rna-interference-rnai-services/in-vivo-sirna-delivery-and-tissue-targeting/
In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi) In Vivo siRNA Delivery and Tissue Targeting. RNAi-induced gene silencing is used to study gene function in cultured cells (in vitro).Most often, these effects are induced by the introduction of small-interfering RNA (siRNA) or microRNA (miRNA).
https://en.wikipedia.org/wiki/File:ShRNA_Lentivirus.svg
Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts.A copy of the license is included in the section entitled GNU Free Documentation License.
https://www.sigmaaldrich.com/catalog/product/sigma/shrna
Fully customizable shRNA sequence, vector, and lentiviral manufacturing. Select by methods other than puromycin, including neomycin (G418) and FACS via reporters. Confirm your lentiviral delivery through a fluorescent reporter. Customize your shRNA sequence. Eliminate tedious cloning and viral production.
https://www.sigmaaldrich.com/catalog/genes/ABCA1
Human(19) Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White).
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